Anti-Obesity Effect of Pinus densiflora Leaf Extracts

Min Yeong Choi; Byel Shin; Ju Hyeong Yu; Joo Ho Yeo; Jae Won Lee; Na Gyeong Geum; Mi-Yun An; Jin Boo Jeong

doi:10.7732/kjpr.2022.35.2..385

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2022 Vol.35, Issue 2 Preview Page

Research Article

Anti-Obesity Effect of Pinus densiflora Leaf Extracts 솔잎(Pinus densiflora leaf) 추출물의 항비만효과

1 April 2022. pp. 385-389

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Abstract

In this study, we measured the inhibitory activity of Pinus densiflora leaf (PDL) against excessive lipid accumulation in mouse preadipocyte, 3T3-L1 cells to investigate whether PDL exerts anti-obesity activity. Lipid accumulation and the protein level were measured using Oil red O staining assay and Western blot analysis, respectively. We observed that PDL inhibited excessive lipid accumulation and decreased the expression of CEBPα, PPARγ and perilipin-1 related to lipid accumulation in 3T3-L1 cells. Therefore, considering these results, PDL can be used as a potential agent for anti-obesity.

Keywords

Anti-obesity activity

Adipocytes

Pinus densiflora

3T3-L1 cells

References

Ando, Y., F. Sato, H. Fukunaga, Y. Iwasaki, Y. Chiba, M. Tebakari, Y. Daigo, J. Kawashima and J. Kamei. 2019. Placental extract suppresses differentiation of 3T3-L1 preadipocytes to mature adipocytes via accelerated activation of p38 MAPK during the early phase of adipogenesis. Nutr. Metab. 16(1):1-13. 10.1186/s12986-019-0361-831139234PMC6528359

Brun, R.P., J.B. Kim, E. Hu, S. Altiok and B.M. Spiegelman. 1996. Adipocyte differentiation: a transcriptional regulatory cascade. Curr. Opin. Cell Biol. 8(6):826-832. 10.1016/S0955-0674(96)80084-6

Chang, H.C., H.C. Yang, H.Y. Chang, C.J. Yeh, H.H. Chen, K.C. Huang and W.H. Pan. 2017. Morbid obesity in Taiwan: Prevalence, trends, associated social demographics, and lifeꠓstyle factors. PLoS One 12: e0169577. 10.1371/journal.pone.016957728152059PMC5289420

Choi, H.Y. 2009. Antioxidant activity and quality characteristics of pine needle cookies. J. Korean Soc. Food Sci. Nutr. 38:1414-1421. 10.3746/jkfn.2009.38.10.1414

Fajas, L., J.C. Fruchart and J. Auwerx. 1998. Transcriptional control of adipogenesis. Curr. Opin. Cell Biol. 10(2):165-173. 10.1016/S0955-0674(98)80138-5

Ferris, C.D., S.R. Jaffrey, A. Sawa, M. Takahashi, S.D. Brady, R.K. Barrow, A.T Steven, W. Herman, D.E Barañano, S. Doré, K.D Poss and S.H. Snyder. 1999. Heme oxygenase-1 prevents cell death by regulating cellular iron. Nat. Cell Biol. 1(3):152-157. 10.1038/1107210559901

Geum, N.G., H.J. Son, J.H. Yeo, J.H. Yu, M.Y. Choi, J.W. Lee, J.K. Baek and J.B. Jeong. 2021. Anti-obesity activity of Heracleum moellendorffii root extracts in 3T3‐L1 adipocytes. Food Sci. Nutr. 9(11):5939-5945. 10.1002/fsn3.248734760227PMC8565208

Gopalakrishnan, N., M. Saravanakumar, P. Madankumar, M. Thiyagu and H. Devaraj. 2014. Colocalization of β-catenin with Notch intracellular domain in colon cancer: a possible role of Notch1 signaling in activation of Cyclin D1-mediated cell proliferation. Mol. Cell. Biochem. 396(1):281-293. 10.1007/s11010-014-2163-725073953

Inokawa, A., T. Inuzuka, T. Takahara, H. Shibata and M. Maki. 2016. Tubby-like protein superfamily member PLSCR3 functions as a negative regulator of adipogenesis in mouse 3T3-L1 preadipocytes by suppressing induction of late differentiation stage transcription factors. Biosci. Rep. 36(1):e00287. 10.1042/BSR2015021526677203PMC4725246

Ji, H.H., H.Y. Jeong, S. Jin, H.J. Kwon and B.W. Kim. 2012. Inhibition of adipocyte differentiation by methanol extracts of Oenanthe javanica seed in 3T3-L1 preadipocytes. J. Life Sci. 22(12):1688-1696. 10.5352/JLS.2012.22.12.1688

Kim, D.H., S.J. Kim, S.I. Jeong, C.J. Cheon and S.Y. Kim. 2014. Antiadipogenic effects of red radish (Raphanus sativus L.) sprout extract in 3T3-L1 preadipocytes. J. Life Sci. 24(11):1224-1230. 10.5352/JLS.2014.24.11.1224

Kim, E.A., J.H. Yang, E.H. Byeon, W. Kim, D. Kang, J. Han, S.G. Hong, D.R. Kim, S.J. Park, J.W. Huh, H. Cheong, S.P. Yun and D.K. Lee. 2021. Anti-Obesity effect of pine needle extract on high-fat diet-induced obese mice. Plants 10(5):837. 10.3390/plants1005083733919440PMC8143554

Lee, J.E. and M.S. Oh. 2008. Quality characteristics of seasoned sauce and seasoned pork rib with added pine needle powder during storage. Korean. J. Food. Cook Sci. 23(5):629-638.

Lee, M. and S.H. Sung. 2016. Platyphylloside isolated from Betula platyphylla inhibit adipocyte differentiation and induce lipolysis via regulating adipokines including PPARγ in 3T3-L1 cells. Pharmacogn. Mag. 12(48):276.

Li, M., D.H. Kim, P.L. Tsenovoy, S.J. Peterson, R. Rezzani, L.F. Rodella, W.S. Aronow, S. Ikehara and N.G. Abraham. 2008. Treatment of obese diabetic mice with a heme oxygenase inducer reduces visceral and subcutaneous adiposity, increases adiponectin levels, and improves insulin sensitivity and glucose tolerance. Diabetes 57(6):1526-1535. 10.2337/db07-176418375438

Park, J.H. and D.W. Kim. 2006. A study on the short term effect of Rossa rugosae Radix on proliferation, differentiation & maturation of 3T3-L1 preadipocyte. Herb. Formula Sci. 14(2):86-96.

Tontonoz, P., E. Hu and B.M. Spiegelman. 1994. Stimulation of adipogenesis in fibroblasts by PPARγ2, a lipid-activated transcription factor. Cell 79(7):1147-1156. 10.1016/0092-8674(94)90006-X

Yu, J.H., N.G. Geum, J.H. Yeo and J.B. Jeong. 2021. Immuno-enhancing and anti-obesity effect of Abelmoschus manihot root extracts. Korean J. Plant Res. 34(5):411-419.

Yu, W., K.E. Rohli, S. Yang and P. Jia. 2021. Impact of obesity on COVID-19 patients. J. Diabetes Complicat. 35(3):107817. 10.1016/j.jdiacomp.2020.10781733358523PMC7690270

Information

Publisher :The Plant Resources Society of Korea
Publisher(Ko) :한국자원식물학회
Journal Title :Korean Journal of Plant Resources
Journal Title(Ko) :한국자원식물학회지
Volume : 35
No :2
Pages :385-389
Received Date : 2022-02-11
Revised Date : 2022-03-08
Accepted Date : 2022-03-08
DOI :https://doi.org/10.7732/kjpr.2022.35.2..385

Korean Journal of Plant Resources ISSN:1226-3591(Print) 2287-8203(Online) 한국자원식물학회지

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