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2026 Vol.39, Issue 2

Research Article

Anti-Inflammatory Effects and Underlying Mechanisms of the Ethyl Acetate Fraction from Gloeostereum incarnatum Mycelia in LPS-Stimulated RAW264.7 Macrophages

느릅나무버섯(Gloeostereum incarnatum) 균사체 ethyl acetate 분획의 RAW264.7 대식세포에서의 항염증 효과 및 작용기전

Hyeok Jin Choi1†
,
Jeong Won Choi2
,
So Jung Park1
,
Gyeong Eun Im1
,
Bo Hee Seo1
,
Ha Eun Kim3
,
Youngki Park4
,
Kyoung Tae Lee5†
,
Jin Boo Jeong6
최 혁진1†
,
최 정원2
,
박 소정1
,
임 경은1
,
서 보희1
,
김 하은3
,
박 영기4
,
이 경태5†
,
정 진부6
1Graduate Student, Department of Forest Science, Andong National University, Andong 36729, Korea
2Post-doc, Department of Forest Science, Andong National University, Andong 36729, Korea
3Undergraduate Student, Department of Forest Science, Andong National University, Andong 36729, Korea
4Senior Researcher, Department of Forest Bioresources, Division of Forest Microbiology, National Institute of Forest Science, Suwon 16631, Korea
5Researcher, Department of Forest Bioresources, Division of Forest Microbiology, National Institute of Forest Science, Suwon 16631, Korea
6Professor, Department of Forest Science, Andong National University, Andong 36729, Korea
1국립경국대학교 산림과학과, 대학원생
2국립경국대학교 산림과학과, 박사후 연구원
3국립경국대학교 산림과학과, 학부생
4국립산림과학원 산림생명자원연구부, 연구관
5국립산림과학원 산림생명자원연구부, 연구사
6국립경국대학교 산림과학과, 교수
*Corresponding Author
†These authors contributed equally to this work.
1 April 2026. pp. 35-43
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Abstract

This study investigated the anti-inflammatory activity of the ethyl acetate fraction from the mycelia of Gloeostereum incarnatum (GIM-EAF) and its underlying molecular mechanisms in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. Among the solvent fractions obtained from the 70% ethanol extract, GIM-EAF showed the strongest inhibitory effect on NO production. GIM-EAF significantly and dose-dependently suppressed the production of NO and PGE2 without cytotoxicity, accompanied by reduced expression of inducible nitric oxide synthase and cyclooxygenase-2. Mechanistically, GIM-EAF inhibited the phosphorylation of MAPKs, including ERK1/2, p38, and JNK, as well as their downstream target ATF2 and NF-κB p65, indicating suppression of major pro-inflammatory signaling pathways. In contrast, GIM-EAF enhanced PI3K phosphorylation and promoted Nrf2 accumulation and HO-1 expression. These effects were significantly attenuated by the PI3K inhibitor LY294002, suggesting that activation of the PI3K/Nrf2/HO-1 pathway contributes to its anti-inflammatory action. Collectively, these findings suggest that GIM-EAF exerts potent anti-inflammatory effects by downregulating MAPK and NF-κB signaling while activating the PI3K/Nrf2/HO-1 axis in LPS-stimulated macrophages.

Keywords
Anti-inflammation
Gloeostereum incarnatum Mycelia
Macrophages
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 39
  • No :2
  • Pages :35-43
  • Received Date : 2026-03-22
  • Accepted Date : 2026-03-23
  • DOI :https://doi.org/10.7732/kjpr.2026.39.2.035
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