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2025 Vol.38, Issue 6 Preview Page

Research Article

Development of Transcriptome-Based Single Nucleotide Polymorphism Markers for Pear Skin Color
Se Hee Kim1
,
Sewon Oh2
,
Sang-Yun Cho1
,
Hyun Ran Kim3
1Researcher, Fruit Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju 55365, Korea
2Post-doc, Fruit Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju 55365, Korea
3Senior Researcher, Fruit Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju 55365, Korea
*Corresponding Author
1 December 2025. pp. 794-802
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Abstract

Breeding efficiency in new pear cultivar is considerably low due to long juvenility and self-incompatibility. Therefore, it is necessary to develop early selection markers to screen seedlings with target traits and to increase breeding efficiency. For the comparison of transcription profiles in pear cultivars with different skin color, two cDNA libraries were constructed. Differences in gene expression between red-skinned ‘Rosired Bartlett’ and green-skinned ‘Bartlett’ were investigated by NGS. EST of clones from two cultivars was selected for nucleotide sequence determination and homology searches. MYB10, CHI, and F3H were more predominantly expressed in the red-skinned cultivar rather than the green- skinned cultivar by qRT-PCR. Putative SNPs were screened from pear EST contigs by HRM analysis between ‘Rosired Bartlett’ and ‘Bartlett’. All 21 pairs of SNPs were discriminated and the HRM profiles of amplicons were established. In this study, we report an efficient way to develop two SNP markers for distinguishing between red- and green-skinned pear cultivars by HRM analysis. These SNP markers would be useful for pear marker assisted breeding and provide a good reference for relevant research on molecular mechanisms of color variation in pear cultivars.

Keywords
Anthocyanin
Breeding
Cultivars
Gene expression
Molecular marker
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 38
  • No :6
  • Pages :794-802
  • Received Date : 2025-11-13
  • Accepted Date : 2025-11-13
  • DOI :https://doi.org/10.7732/kjpr.2025.38.6.794
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