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2023 Vol.36, Issue 5 Preview Page
Comparison of Regeneration Conditions in Seven Pepper (Capsicum annuum L.) Varieties

7종의 고추(Capsicum annuum L.) 재분화 조건 비교

Min-Su Kim1†
,
Yun-Jeong Han2†
,
Sharanya Tripathi1
,
Jinwoo Kwak1
,
Jin-Kyung Kwon3
,
Byoung-Cheorl Kang4
,
Jeong-Il Kim56*
김 민수1†
,
한 윤정2†
,
트리파티 샤란야1
,
곽 진우1
,
권 진경3
,
강 병철4
,
김 정일56*
1Graduate Students, Department of Integrative Food, Bioscience and Biotechnology, Chonnam National University, Gwangju 61186, Korea
2Academic Research Professor, Kumho Life Science Laboratory, Chonnam National University, Gwangju 61186, Korea
3Research Professor, Department of Agriculture, Forestry and Bioresources & Plant Genomics and Breeding Institute, Seoul National University, Seoul 08826, Korea
4Professor, Department of Agriculture, Forestry and Bioresources & Plant Genomics and Breeding Institute, Seoul National University, Seoul 08826, Korea
5Professor, Department of Integrative Food, Bioscience and Biotechnology, Chonnam National University, Gwangju 61186, Korea
6Director, Kumho Life Science Laboratory, Chonnam National University, Gwangju 61186, Korea
1전남대학교 융합식품바이오공학과, 대학원생
2전남대학교 금호생명과학연구소, 연구교수
3서울대학교 농림생물자원학부 및 식물유전체육종연구소, 연구교수
4서울대학교 농림생물자원학부 및 식물유전체육종연구소, 교수
5전남대학교 융합식품바이오공학과, 교수
6전남대학교 금호생명과학연구소, 교수
*Corresponding Author
†These authors contributed equally to this work.
1 October 2023. pp. 527-539
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Abstract

Pepper (Capsicum annuum L.) is an important vegetable and spice crop that has been cultivated worldwide. Pepper fruits have unique taste and aroma, providing a variety of antioxidants and compounds important for human health, which makes a high economic value. In addition, there is a high demand for new pepper varieties, according to consumer’s preference. However, pepper is a recalcitrant plant for in vitro tissue and organ differentiation and plant regeneration, which makes it difficult to develop demanded varieties using newly developed technologies such as genetic engineering and gene editing. In this study, tissue culture and regeneration conditions were investigated using seven pepper varieties that were obtained from the core-collection of Seoul National University. We observed callus and bud induction and shoot formation using several media composition composed of different cytokinins and auxin concentrations. As a result, it was found that there were differences in callus induction and shoot formation of each variety depending on the hormone composition, and the highest regeneration was shown when the medium containing Zeatin Riboside and the petioles of seedlings were used. In particular, out of seven pepper varieties, CMV980 exhibited a higher regeneration efficiency (approximately 48%) than other varieties, followed by Yuwolcho. Therefore, this study provides CMV980 and Yuwolcho as good candidates that can be used for pepper transformation, which might contribute to the development of various varieties through gene editing technology in the future.

Keywords
Capsicum annuum
CMV980
Pepper
Regeneration
Yuwolcho
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 36
  • No :5
  • Pages :527-539
  • Received Date : 2023-08-18
  • Revised Date : 2023-09-04
  • Accepted Date : 2023-09-04
  • DOI :https://doi.org/10.7732/kjpr.2023.36.5.527
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