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2021 Vol.34, Issue 6 Preview Page

Research Article

Efficient Cryopreservation of In Vitro Grown Shoot Tips of Strawberry (Fragaria x ananassa Duch.) Germplasm Using Droplet-Vitrification
Jinjoo Bae1
,
Sun-Yi Lee2
,
Jae-Young Song1
,
Jung-Ro Lee3
,
Munsup Yoon3
,
Jung-Yoon Yi3
,
Haeng-Hoon Kim4
,
Young-Yi Lee5*
1Post-doc, National Agrobiodiversity Center, NAS, RDA, Suwon 57922, Korea
2Researcher, Department of Horticultural Crop Research, NIHHS, RDA, Wanju 55365, Korea
3Senior Researcher, National Agrobiodiversity Center, NAS, RDA, Suwon 57922, Korea
4Professor, Department of Well-being Resources, Sunchon National University, Suncheon 57922, Korea
5Researcher, National Agrobiodiversity Center, NAS, RDA, Suwon 57922, Korea
*Corresponding Author
1 December 2021. pp. 600-607
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Abstract

Cryopreservation method using a droplet vitrification was applied to the thirty-one strawberry accessions of in vitro grown shoot tips. A protocol with 0.3 - 0.5 M preculture followed by C4 loading and B1 dehydration solutions efficiently implemented cryopreservation of twenty-six strawberry accessions. The highest regrowth rate was 85.8% for PHS0007 and others were ranged between 85.8% and 21.0%. A slightly modified protocol was applied to five accessions. With these two protocols, twenty-eight accessions obtained more than 40% regrowth rate. This study showed that the droplet vitrification method was able to practically implement cryopreservation of in vitro grown shoot tips of broad range of strawberry germplasm (105).

Keywords
Cryopreservation
Droplet-vitrification
Shoot tip
Strawberry
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 34
  • No :6
  • Pages :600-607
  • Received Date : 2021-10-31
  • Revised Date : 2021-11-11
  • Accepted Date : 2021-11-15
  • DOI :https://doi.org/10.7732/kjpr.2021.34.6.600
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