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2025 Vol.38, Issue 6 Preview Page

Research Article

Optimization of Adventitious Root Culture Conditions and Airlift Bioreactor Scale-Up in Four Jeju Native Plants
Jong-Du Lee15
,
Eunbi Jang15
,
Weon-Jong Yoon2
,
Young Min Ham2
,
Ji-Gweon Park3
,
So-Young Park4
,
Yong-Hwan Jung3
1Researcher, Biodiversity Research Institute, Clean Bio Business Division, Jeju Technopark, Jeju 63608, Korea
2Principal Researcher, Biodiversity Research Institute, Clean Bio Business Division, Jeju Technopark, Jeju 63608, Korea
3Senior Researcher, Biodiversity Research Institute, Clean Bio Business Division, Jeju Technopark, Jeju 63608, Korea
4Professor, Department of Horticultural Science, Chungbuk National University, Cheongju 28644, Korea
5Graduate Student, Department of Horticultural Science, Chungbuk National University, Cheongju 28644, Korea
*Corresponding Author
1 December 2025. pp. 759-770
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Abstract

Jeju Island, a UNESCO World Natural Heritage Site, is rich in native plant diversity. However, many of these plants are underutilized due to propagation challenges and ecological sensitivity. In line with international biodiversity frameworks (CBD and ABS), this study aimed to establish an optimized in vitro adventitious root (AR) culture platform for the conservation and bioutilization of selected Jeju native plants. Extensive surveys and in vitro evaluations identified four high-performance candidates for AR induction, Calystegia soldanella (CAS), Hibiscus hamabo (HIH), Lycoris chejuensis (LYC), and Tetragonia tetragonoides (TET). We systematically optimized culture conditions, finding that Murashige and Skoog (MS) media and specific auxin treatments (IBA and NAA) enhanced rooting efficiency. The most responsive cultures were scaled up in 5 L airlift bioreactors. Biomass performance was quantified by various metrics, including growth index and relative growth rate. CAS and HIH showed superior proliferation, yielding over 30 g DW·batch-1 with a relative growth rate above 3.0. This study successfully optimized a bioreactor-based AR culture platform, demonstrating its technical and biological feasibility for both ex situ conservation and industrial-scale bioproduction. The platform provides a scalable, ESG-aligned model for the genetic preservation and sustainable valorization of Jeju’s unique botanical resources within the emerging bioeconomy.

Keywords
Auxins, Biomass yield
Ex situ conservation
In vitro propagation
Plant cell line bank
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 38
  • No :6
  • Pages :759-770
  • Received Date : 2025-09-03
  • Revised Date : 2025-10-20
  • Accepted Date : 2025-10-23
  • DOI :https://doi.org/10.7732/kjpr.2025.38.6.759
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