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2023 Vol.36, Issue 4
Induction of Autophagy by Rosa acicularis Leaves Extracts in RAW264.7 Cells

인가목(Rosa acicularis Lindl.) 잎 추출물의 대식세포에서 자가포식 유도활성

Jeong Won Choi1
,
Jin Boo Jeong2*
최 정원1
,
정 진부2*
1Graduate Student, Department of Medicinal Plant Resources, Andong National University, Andong 36729, Korea
2Professor, Department of Medicinal Plant Resources, Andong National University, Andong 36729, Korea
1국립안동대학교 생약자원학과, 대학원생
2국립안동대학교 생약자원학과, 교수
*Corresponding Author
1 August 2023. pp. 257-263
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Abstract

Autophagy contributes to enhancing the immune system (innate and adaptive immune system) against foreign pathogens. Autophagy of macrophages is used as a major indicator for developing vaccine adjuvants to increase the adaptive immune response. In this study, water extracts from Rosa acicularis leaves (RAL) increased the production of immunostimulatory mediators and phagocytic activity in RAW264.7 cells. RAL increased p62/SQSTM1 expression. Inhibition of TLR4, JNK, and PI3K/AKT blocked RAL-mediated increase of p62/SQSTM1. RAL activated JNK and PI3K/AKT signaling. RAL-mediated activations of JNK and PI3K/AKT signaling were reversed by TLR4 inhibition. Taken together, it is believed that RAL-mediated autophagy may be dependent on activating via TLR4-dependent activation of JNK and PI3K/AKT signaling in macrophages.

Keywords
Autophagy
Immunostimulation
Macrophages
Rosa acicularis Lindl.
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 36
  • No :4
  • Pages :257-263
  • Received Date : 2023-01-19
  • Revised Date : 2023-05-09
  • Accepted Date : 2023-05-15
  • DOI :https://doi.org/10.7732/kjpr.2023.36.4.257
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