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2018 Vol.31, Issue 2 Preview Page

Research Article

Anti-colorectal Cancer and Anti-oxidant Activities of Rubiae radix Ethanol Extract in vitro

천초근 에탄올 추출물의 항산화 효능 및 대장암 세포 억제 효과

Jong Hyun Nho1*
,
Mi Ok Sim1*
,
Ho Kyung Jung1
,
Mu Jin Lee1
,
Ji Hun Jang1
,
Da Eun Jung1
,
Tae Kyoung Sung1
,
Byeong Kwan An1
,
Hyun Woo Cho1**
노 종현1*
,
심 미옥1*
,
정 호경1
,
이 무진1
,
장 지훈1
,
정 다은1
,
성 태경1
,
안 병관1
,
조 현우1**
1National Development Institute of Korean Medicine, Jangheung-gun 59338, Korea
1한약진흥재단
*First Author
** Corresponding Author
30 April 2018. pp. 102-108
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Abstract

Rubiae radix is root of Runia akane Nakai, it has been used to hemostasis and blood stasis in Korean and China. This study investigated that anti-oxidant and anti-colorectal cancer effect of ERA (ethanol extract of Rubiae radix) and WRA (water extract of Rubiae radix) using RAW 264.7 (murine macrophage from blood) and HCT-116 cells (human colorectal cancer cell line). ERA contained polyphenol (45.77 ± 2.03 ㎎/g) and flavonoid (22.82 ± 1.33 ㎎/g). 500 μM H2O2-induced ROS generation was diminished by 500 ㎍/㎖ ERA treatment in RAW 264.7 cells, but not WRA (125, 250, and 500 ㎍/㎖). Moreover, caspase-3 activity and DNA fragmentation increased by 500 ㎍/㎖ ERA treatment during apoptotic cell death in HCT-116. Results demonstrated that anti-cancer effect of ERA against human colorectal cancer cells is mediated apoptotic cell death and DNA fragmentation through caspase-3 activation. However, further study is required to what active ingredient of ERA are important for anti-oxidant and anti-colorectal cancer effect in vivo.

Keywords
Anti-oxidative effect
Colorectal cancer
Rubia akane
Nakai
Rubiae radix
References
1
Andersen, C., L. Adamsen, T. Moeller, J. Midtgaard, M. Quist, A. Tveteraas and M. Rorth. 2006. The effect of a multidimensional exercise programme on symptoms and side-effects in cancer patients undergoing chemotherapy-the use of semi-structured diaries. Eur. J. Oncol. Nurs. 10:247-262.
10.1016/j.ejon.2005.12.00716476570
2
Anwar, F., S. Latif, M. Ashraf and A.H. Gilarni. 2007. Moringa oleifera : a food plant with multiple medicinal uses. Phytother. Res. 21:17-25.
10.1002/ptr.202317089328
3
Bae, J.H., H.J. Jang and J.I. Jung. 2005. Antimicrobial effect of Rubia akane Nakai on food-borne pathogens. J. Korean Soc. Food Sci. Nutr. 34:389-394.
10.3746/jkfn.2005.34.3.389
4
Choi, H.S., J.M. Park, S.M. Ju, S.H. Kim, D.K. Kim, W.S. Kim and B.H. Jeon. 2008. Influence of Rubiae radix extract on the mechanism of apoptosis in HL-60 cells. J. Physiol and Pathol. Korean Med. 22:548-555.
5
Folin, O. and W. Denis. 1912. On phosphotungstic-phosphomolybdic compounds as color reagents. J. Biol. Chem. 12:239-243.
6
Horakova, L. 2011. Flavonoids in prevention of diseases with respect to modulation of Ca-pump function. Interdiscip. Toxicol. 4:114-24.
10.2478/v10102-011-0019-522058652 PMC3203913
7
Jeong, J.H., W.H. Hwang, S.H. An, H.Y. Jeong, H.S. Lee, J.S. Baek, K.J. Choi, G.H. Lee, J.E. R, N.J. Chung, S.J. Lee and S.J. Yun. 2017. Seed germination, plant growth and antioxidant capacity of Limonium tetragonum under different salt concentrations. Korean J. Plant Res. 30:364-371.
8
Kitazumi, I. and M. Tsukahara. 2010. Regulation of DNA fragmentation: the role of caspases and phosphorylation. FEBS J. 278-427-441.
9
Kwon, H.N., W.S. Bang, J.Y. Kim, J.R. Park and J.R. Jeon. 2011. Effect of Acer tegmentosum M. extracts on hepatocarcinoma cell. Korean J. Food Sci. Technol. 43:787-790.
10.9721/KJFST.2011.43.6.787
10
Kwon, H.N., W.S. Bang, J.Y. Kim, J.R. Park and J.R. Jeon. 2011. Effect of Acer tegmentosum M. extracts on hepatocarcinoma cell. Korean J. Food Sci. Technol. 43:787-790.
10.9721/KJFST.2011.43.6.787
11
Kim, J.Y., E.J. Jung, Y.S. Won, J.H. Lee, D.Y. Shin and K.I. Seo. 2012a. Cultivated Orostachys japonicus induces apoptosis in human colon cancer cells. Korean J. Food Sci. Technol. 44:317-323.
10.9721/KJFST.2012.44.3.317
12
Kim, D.H., J.H. Park, S.H. Kim and S.H. Sung. 2012b. Case study: regression of a residual tumor and prolongation of overall survival with allergen-removed Rhus verniciflua stokes after chemoradiotherapy in locally advanced non-small cell lung cancer. Korean J. Intern. Med. 36:200-206.
13
Kim, Y.Z., J.H. Kim, J.Y. Lee, S.E. Park, J.M. Choi and Y.G. Park. 2013. symptoms and initial diagnostic test in colorectal cancer patients. Korean J. Fam. Pract. 3:58-64.
14
Kwon, Y.R. and K.S. Youn. 2014. Antioxidant activity and physiological properties of moringa (Moringa oleifera Lam.) leaves extracts with different solvents. Korean J. Food Preserv. 21:831-837.
10.11002/kjfp.2014.21.6.831
15
Kim, B.M., G.T. Kim, E.G. Lim, E.J. Kim, S.Y. Kim, S.H. Ha and Y.M. Kim. 2015. Cell cycle arrest of extract from Artemisia annua Linne via Akt-mTOR signaling pathway in HCT116 colon cancer cells. Korean Soc. Biotechnol. Bioeng. J. 30:223-229.
10.7841/ksbbj.2015.30.5.223
16
Kim, J.H. and E.J. Kim. 2018. Evaluation of anti-oxidative, anti-thrombin, anti-invasive and pro-apoptotic activities of Paeonia japonica. Korean J. Plant Res. 31:16-23.
17
Lee, S.E., J.H. Choi, J.H. Lee, H.J. Noh, G.S. Kim, J.K. Kim, H.Y. Chung and S.Y. Kim. 2013. Screening of useful plants with anti-inflammatory and antioxidant activity. Korean J. Plant Res. 26:441-449.
10.7732/kjpr.2013.26.4.441
18
Lewis, J.K. and W. Addison. 2015. Principles of cancer biology. San Francisco, USA. pp. 11-30.
19
Moreno, M.I., M.I. Isla, A.R. Sampietro and M.A. Vattuone. 2000. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J. Ethnopharmacol. 71:109-114.
10.1016/S0378-8741(99)00189-0
20
Pandey, K.B. and S.I. rizvi. 2009. Plant polyphenols as dietary antioxidants in human disease. Dxid. Med. Cell Longev. 2:270-278.
10.4161/oxim.2.5.949820716914PMC2835915
21
Rhee, S.G. 2006. Cell signaling. H2O2, a necessary evil for cell signlaing. Science 312:1182-1183.
10.1126/science.113048116809515
22
Shin, Y.J., D.Y. Jung, H.K. Ha and S.W. Park. 2004. Anticancer effect of Erythronium japonicum extract on ICR mouse and L1210 cells with alteration of antioxidant enzyme activities. Korean J. Food Sci. Technol. 36:968-973.
23
Singh, N.P. and H.C. Lai. 2004. Artermisinin induces apoptosis in human cancer cells. Anticancer Res. 24:2277-2280.
15330172
24
Shariati, A., S. Haghighi, S. Fayyazi, H. Tabesh and M.M. Kalboland. 2010. The effect of exercise on the severity of the fatigue in colorectal cancer patients who received chemotherapy in ahwaz. Iran J. Nurs. Midwifery Res. 15:145-149.
21589787PMC3093180
25
Sim, M.O., H.J. Lee, J.H. Jang, H.E. Lee, H.K. Jung, T.M. Kim, J.H. No, J.K. Jung and H.W. Cho. 2017. Anti-inflammatory and antioxidant effects of Spiraea prunifolia Sieb. et Zucc. var. simpliciflora Nakai in RAW 264.7 cells. Korean J. Plant Res. 30:335-342.
26
Woo, K.W., M.O. Sim, H. Bak, H.K. Jung, B.K. An, S.H. Ham, J.H. Park and H.W. Cho. 2018. Protective effects of flavonoids from Boehmeria quelpaertense against H2O2-induced cytotoxicity in H9c2 cardiomyblast cells. Korean J. Plant Res. 31:1-9.
27
Yu, J.M and H.I. Moon. 2018. Antioxidants and acetyl-cholinesterase inhibitory activity of solvent fractions extracts from Dendropanax morbiferus. Korean J. Plant Res. 30:335-342.
28
Zhang, J.H. and M. Xu 2000. DNA fragmentation in apoptosis. Cell Res. 10:205-211.
10.1038/sj.cr.729004911032172
Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 31
  • No :2
  • Pages :102-108
  • Received Date : 2017-09-22
  • Revised Date : 2017-12-11
  • Accepted Date : 2018-01-19
  • DOI :https://doi.org/10.7732/kjpr.2018.31.2.102
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