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2026 Vol.39, Issue 1 Preview Page

Research Article

Comparative Analysis of Antioxidant and Anti-inflammatory Activities of Okhwang 1ho (Abeliophyllum distichum Nakai) Flower Extracts Prepared with Different Solvents

미선나무 (Abeliophyllum distichum Nakai, 옥황1호) 꽃 용매별 추출물의 항산화 및 항염증 활성 평가

Yong-Shin Kim1
,
Jae Ho Park2
김 용신1
,
박 재호2
1Professor, Department of Beauty Medical, Jungwon University, Goesan, 28027, Korea
2Professor, Department of Bioindustry, Jungwon University, Goesan 28027, Korea
1중원대학교 뷰티메디컬학과, 교수
2중원대학교 바이오산업학과, 교수
*Corresponding Author
1 February 2026. pp. 10-18
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Abstract

This study examined the solvent-dependent antioxidant and anti-inflammatory activities of Okhwang 1ho (Abeliophyllum distichum) flower extracts prepared with varying ethanol concentrations (EFP-0, EFP-30, EFP-50, and EFP-100). Radical scavenging capacity was assessed via DPPH and ABTS assays (50~200 ㎍/mL). All extracts demonstrated marked antioxidant activity at low concentrations, while EFP-30 and EFP-50 exhibited superior activity, implying efficient extraction of moderately polar phenolic constituents. Total phenolic content was highest in EFP-50, whereas total flavonoid content increased with ethanol concentration. HPLC profiling confirmed distinct phytochemical signatures: EFP-0 contained rutin, caffeic acid, and acteoside, while EFP-30 and EFP-50 additionally contained isoquercitrin and isoacteoside. In RAW 264.7 macrophages, no cytotoxicity was observed up to 200 ㎍/mL. Under LPS-induced inflammatory conditions, EFP-50 and EFP-100 significantly suppressed NO production, consistent with phenolic/flavonoid enrichment. Western blot analysis further verified downregulation of iNOS and COX-2 expression, indicating inhibition of inflammatory signaling. Collectively, these findings demonstrate that the biological activities of A. distichum flower extracts are strongly influenced by solvent polarity, with 30~50% ethanol yielding optimal bioactive profiles. The study supports the potential application of A. distichum flowers as functional ingredients in health-promoting products.

Keywords
Abeliophyllum distichum
Anti-inflammatory activity
Antioxidant activity
Okhwang 1ho
Polyphenols
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 39
  • No :1
  • Pages :10-18
  • Received Date : 2026-01-19
  • Accepted Date : 2026-01-19
  • DOI :https://doi.org/10.7732/kjpr.2026.39.1.010
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