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2026 Vol.39, Issue 3 Preview Page
In vitro Callus Induction from Root Tip and Bulb Meristem Explants of Two Ornamental Allium Species Native to Korea
Jeong Min Kim1†
,
Chung Hwa Park1†
,
Ji Young Jung2
,
Chung Ho Ko2
,
Bo-Kook Jang3
1Post-Mas, Forest Biological Resources Utilization Center, Korea National Arboretum, Yangpyeong 12519, Korea
2Researcher, Forest Biological Resources Utilization Center, Korea National Arboretum, Yangpyeong 12519, Korea
3Assistant Professor, Department of Horticulture, Sunchon National University, Suncheon 57922, Korea
*Corresponding Author
†These authors contributed equally to this work.
1 June 2026. pp. 162-169
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Abstract

Successful in vitro micropropagation of two ornamental Allium species native to Korea, A. dumebuchum H.J.Choi and A. thunbergii G.Don, depends on establishing suitable culture conditions for callus induction. However, the types and concentrations of plant growth regulator (PGR) required for callus induction in ornamental Allium species remain poorly understood. In this study, bulb meristems and seed-derived root tip explants were cultured on Murashige and Skoog media supplemented with 0.1, 0.5, or 1.0 ㎎·L-1 1-naphthaleneacetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), 6-benzylaminopurine (BAP), or thidiazuron (TDZ) to determine species- and explant-specific requirements for callus induction in A. dumebuchum and A. thunbergii. Callus induction and root regeneration were evaluated after 3 months of culture. In both species, callus was successfully induced from root tip explants, whereas bulb meristem cultures showed signs of contamination. In A. dumebuchum, the highest callus induction frequencies were observed in root tip and bulb meristem explants cultured on 1.0 ㎎·L-1 NAA (90.0%) and 0.1–1.0 ㎎·L-1 BAP (100.0%), respectively. In A. thunbergii, root tip explants cultured on 0.1 ㎎·L-1 2,4-D showed the highest callus induction frequency (100.0%), whereas bulb meristem explants formed callus only on media supplemented with 0.5 ㎎·L-1 BAP or 0.5–1.0 ㎎·L-1 TDZ. These results suggest that seed-derived root tip explants are a more practical starting material for aseptic callus culture establishment in both species under the present culture conditions. The most effective PGR treatment for root tip-derived callus induction differed between the two species: 1.0 ㎎·L-1 NAA for A. dumebuchum and 0.1 ㎎·L-1 2,4-D for A. thunbergii.

Keywords
Allium dumebuchum H.J.Choi
Allium thunbergii G.Don
Bulb meristem
Plant growth regulator
Root tip explant
References
1

Bai, X., H.-S. Lee, H.N. Murthy, H.-J. Kwon, S.-H. Yeon, J.-Y. Ju, and S.-Y. Park. 2024. Micropropagation of Lobelia chinensis Lour.: Influence of medium parameters on plant regeneration, antioxidant activity, and secondary metabolite accumulation. Korean J. Plant Res. 37:225-234.

10.7732/KJPR.2024.37.3.225
2

Barandiaran, X., N. Martín, M.F. Rodríguez-Conde, A. Di Pietro, and J. Martín. 1999. Genetic variability in callus formation and regeneration of garlic (Allium sativum L.). Plant Cell Rep. 18:434-437.

10.1007/s002990050599
3

Buiteveld, J., P. van der Valk, J. Jansen, J. Creemers-Molenaar, and C.M. Colijn-Hooymans. 1993. Callus induction and plant regeneration from explants of commercial cultivars of leek (Allium ampeloprasum var. porrum L.). Plant Cell Rep. 12:431-434.

10.1007/BF00234707
4

Farhadi, N., J. Panahandeh, A.M. Azar, and S.A. Salte. 2017. Effects of explant type, growth regulators and light intensity on callus induction and plant regeneration in four ecotypes of Persian shallot (Allium hirtifolium). Sci. Hortic. 218:80-86.

10.1016/j.scienta.2016.11.056
5

Fritsch, R.M. 2015. Checklist of ornamental Allium species and cultivars currently offered in the trade. Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung Gatersleben, Germany.

6

Hol, G.M.G.M. and P.C.G. van der Linde. 1992. Reduction of contamination in bulb-explant cultures of Narcissus by a hot-water treatment of parent bulbs. Plant Cell Tissue Organ Cult. 31:75-79.

10.1007/BF00043478
7

Jalilian, F., A. Maroufi, and A. Mozafari. 2025. In vitro callus induction and shoot regeneration of an important medicinal plant Stachys inflata Benth. Genet. Resour. Crop Evol. 72:8841-8853.

10.1007/s10722-025-02476-y
8

Jang, J.E., J.S. Park, J.Y. Jung, D.K. Kim, S. Yang, and H.J. Choi. 2021. Notes on Allium section Rhizirideum (Amaryllidaceae) in South Korea and northeastern China: with a new species from Ulleungdo Island. PhytoKeys 176:1-19.

10.3897/phytokeys.176.6337833958935PMC8065018
9

Jang, J.E., S. Baasanmunkh, N. Nyamgerel, S.Y. Oh, J.H. Song, Z. Yusupov, K. Tojibaev, and H.J. Choi. 2024. Flower morphology of Allium (Amaryllidaceae) and its systematic significance. Plant Divers. 46:3-27.

10.1016/j.pld.2023.06.00938343591PMC10851308
10

Leelavathy, S. and P.D. Sankar. 2016. Curbing the menace of contamination in plant tissue culture. J. Pure Appl. Microbiol. 10:2145-2152.

10.22207/JPAM.10.3.54
11

Li, D., C. Ji, L. Shao, T. Xu, X. Chen, X. Wang, R. Zhang, Y. Cui, J. Zhang, and Y. Xia. 2025. An efficient and environmentally friendly strategy for embryogenic callus induction and plant regeneration of Iris pseudacorus L. J. Plant Growth Regul. 44:4541-4555.

10.1007/s00344-025-11705-8
12

Luciani, G.F., A.K. Mary, C. Pellegrini, and N.R. Curvetto. 2006. Effects of explants and growth regulators in garlic callus formation and plant regeneration. Plant Cell Tissue Organ Cult. 87:139-143.

10.1007/s11240-006-9148-5
13

Mosa, K.A., M.A. Alhammadi, N.A. Namdari, M.A. Amin, H.I. Almuhairi, S.S.M. Soliman, A. El-Keblawy, and A. Elnaggar. 2025. Callus induction and comparative GC-MS analysis of calli secondary metabolites derived from leaf, stem and root explants of Tephrosia apollinea (Delile) DC. Plant Cell Tissue Organ Cult. 162:25.

10.1007/s11240-025-03128-w
14

Mostafa, H.H.A., H. Wang, J. Song, and X. Li. 2020. Effects of genotypes and explants on garlic callus production and endogenous hormones. Sci. Rep. 10:4867.

10.1038/s41598-020-61564-432184427PMC7078195
15

Pan, H., R. Liao, Y. Zhang, M. Arif, Y. Zhang, S. Zhang, Y. Wang, P. Zhao, Z. Wang, B. Han, and C. Song. 2024 Establishment of callus induction and plantlet regeneration systems of Peucedanum Praeruptorum dunn based on the tissue culture method. Plant Methods 20:174.

10.1186/s13007-024-01300-539548586PMC11568572
16

Robledo-Paz, A., V.M. Villalobos-Arámbula, and A.E. Jofre-Garfias. 2000. Efficient plant regeneration of garlic (Allium sativum L.) by root-tip culture. In Vitro Cell. Dev. Biol. Plant 36:416-419.

10.1007/s11627-000-0075-6
17

Seo, M.-S., S. Park, K. Ha, and K. Shin. 2025. Utilization of plant in vitro culture for the production of secondary metabolites. Korean J. Plant Res. 38:505-516.

10.7732/KJPR.2025.38.5.505
18

Tubić, L., G. Anačkov, J. Milojević, N. Ghalawenji, N. Mitić, R. Igić, and S. Zdravković-Korać. 2014. High variability in the tissue culture response of root-tips of Allium ascalonicum individuals and optimization of the regeneration procedure. Plant Cell Tissue Organ Cult. 118:101-110.

10.1007/s11240-014-0465-9
19

Zhang, X., M. Lu, M. Li, H. Zhang, L. Yu, and T. Wu. 2025. Efficient callus induction and regeneration of tea plant (Camellia sinensis). Sci. Rep. 15:26848.

10.1038/s41598-025-12271-540702144PMC12287268
20

Zheng, S., B. Henken, E. Sofiari, E. Jacobsen, F.A. Krens, and C. Kik. 1998. Factors influencing induction, propagation and regeneration of mature zygotic embryo-derived callus from Allium cepa. Plant Cell Tissue Organ Cult. 53:99-105.

10.1023/A:1006034623942
Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 39
  • No :3
  • Pages :162-169
  • Received Date : 2026-04-06
  • Revised Date : 2026-04-22
  • Accepted Date : 2026-04-23
  • DOI :https://doi.org/10.7732/kjpr.2026.39.3.162
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