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2024 Vol.37, Issue 3 Preview Page

Research Article

Rosa acicularis Leaves Exert Anti-Obesity Activity through AMPK-Dependent Lipolysis and Thermogenesis in Mouse Adipocytes, 3T3-L1 Cells
Jeong Won Choi1
,
Hyeok Jin Choi1
,
Jin Boo Jeong2
1Graduate Student, Department of Forest Science, Andong National University, Andong 36729, Korea
2Professor, Department of Forest Science, Andong National University, Andong 36729, Korea
*Corresponding Author
1 June 2024. pp. 247-255
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Abstract

In this study, we aimed to verify the anti-obesity activity of R. acicularis leaves (RAL) and elucidate its mechanism of action in 3T3-L1 preadipocytes. RAL dose-dependently inhibited the accumulation of lipid droplets and triacylglycerol. RAL did not affect cell proliferation and survival in undifferentiated 3T3-L1 cells, but it inhibited cell proliferation in differentiating 3T3-L1 cells. RAL increased ATGL, p-HSL, and HSL, and decreased perilipin-1 in differentiating 3T3-L1 cells. In addition, RAL reduced lipid droplet accumulation and increased free glycerol content in differentiated 3T3-L1 cells. RAL increased ATGL and HSL in differentiated 3T3-L1 cells. Also, RAL increased p-AMPK, PPARγ, UCP-1, and PGC-1α in differentiating 3T3-L1 cells. AMPK inhibition by compound C attenuated RAL-mediated increase of ATGL, HSL, PPARγ, and UCP-1 in 3T3-L1 cells. Taken together, it is thought that RAL may inhibit lipid accumulation through lipolysis and thermogenesis via the activation of AMPK in adipocytes.

Keywords
Adipocytes
Anti-obesity activity
Lipolysis
Rosa acicularis
Thermogenesis
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 37
  • No :3
  • Pages :247-255
  • Received Date : 2024-01-29
  • Accepted Date : 2024-02-13
  • DOI :https://doi.org/10.7732/kjpr.2024.37.3.247
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