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2023 Vol.36, Issue 4 Preview Page
Development of Chloroplast Genome-based Insertion/Deletion Markers in the Genus Broussonetia

닥나무 속 식물의 엽록체 유전체 기반 InDel 마커의 개발

Eun Jee Lee1†
,
Yoon A Kim1†
,
Mi Sun Lee1
,
Ju Hyeok Kim1
,
Young Kyu Choi2
,
Jung Sung Kim3
,
Chang Seob Sin3
,
Yi Lee4*
이 은지1†
,
김 윤아1†
,
이 미선1
,
김 주혁1
,
최 용규2
,
김 정성3
,
신 창섭3
,
이 이4*
1Student, Department of Industrial Plant Science & Technology, Chungbuk National University, Cheongju 28644, Korea
2Student, Department of Forest Science, Chungbuk National University, Cheongju 28644, Korea
3Professor, Department of Forest Science, Chungbuk National University, Cheongju 28644, Korea
4Professor, Department of Industrial Plant Science & Technology, Chungbuk National University, Cheongju 28644, Korea
1충북대학교 특용식물학과, 학생
2충북대학교 산림학과, 학생
3충북대학교 산림학과, 교수
4충북대학교 특용식물학과, 교수
*Corresponding Author
†These authors contributed equally to this work.
1 August 2023. pp. 290-298
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Abstract

Several members of the genus Broussonetia are woody plants with high-quality cellulose fibers and are used to make a traditional type of Korean paper known as Hanji. Three of these species, Broussonetia kazinoki, Broussonetia monoica, and Broussonetia papyrifera, are found in the Korean Peninsula. Because it is challenging to distinguish different Broussonetia species based on morphology alone, we have developed a set of insertion/deletion (InDel) markers for genetic identification of these species. From twenty-two Broussonetia samples collected throughout Korea, we selected six for next-generation sequencing analysis. InDel marker candidates were identified by comparing this sequence information with the B. kazinoki chloroplast genome sequence. The marker candidates were used to screen the genomes of the twenty-two Broussonetia plants, and five useful chloroplast-based InDel markers were identified. Detailed genotyping using these five markers showed that the twenty-two plants of the genus Broussonetia could be clustered into five groups, verifying that the markers developed here can be used for breeding, identification, and analysis of species in the genus Broussonetia.

Keywords
Broussonetia kazinoki
Broussonetia monoica
Broussonetia papyrifera
Genotyping
Next generation sequencing
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 36
  • No :4
  • Pages :290-298
  • Received Date : 2022-11-01
  • Revised Date : 2023-03-30
  • Accepted Date : 2023-04-28
  • DOI :https://doi.org/10.7732/kjpr.2023.36.4.290
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