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2024 Vol.37, Issue 4 Preview Page

Research Article

The Effect of Plant Growth Regulators on Regeneration Rate during Tissue Culture of Hop (Humulus lupulus L.)

홉(Humulus lupulus L.) 조직배양 시 재분화율에 미치는 식물생장조절제의 영향

Tae Hyun Ha1
,
Jun-Hyung Lee1
,
So Young Yi2
,
Si-Yong Kang23
하 태현1
,
이 준형1
,
이 소영2
,
강 시용23
1Graduate Student, Department of Horticulture, Kongju National University, Yesan 32439, Korea
2Research Professor, Research Center of Crop Breeding for Omics and Artificial Intelligence, Kongju National University, Yesan 32439, Korea
3Professor, Department of Horticulture, Kongju National University, Yesan 32439, Korea
1공주대학교 산업과학대학 원예학과, 대학원생
2공주대학교 작물오믹스지능육종연구센터, 연구교수
3공주대학교 산업과학대학 원예학과, 교수
*Corresponding Author
1 August 2024. pp. 431-438
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Abstract

Hops enhance beer flavor and bitterness, acting as a preservative. In recent years, the booming trend of craft beer has prompted the introduction of foreign hop varieties for cultivation and production in South Korea. This study focuses on developing efficient in vitro culture condition of the hop (Humulus lupulus L.) variety ‘Cascade’ for treatments of plant growth regulators, i.e. IAA and Cytokinin. Using Auxin IAA and Cytokinin 2iP, Zeatin, BAP, and TDZ on MS medium as plant growth regulators, the experiment involved removing three nodes from the shoot apex. In vitro hop culture showed the highest shoot proliferation rate when only IAA was added, with approximately 21% higher compared to the combination with Cytokinin. Notably, IAA 0.1 ㎎/L + BAP 1 ㎎/L resulted in a superior shoot proliferation rate of around 91%. IAA 0.1 ㎎/L + BAP 1 ㎎/L was advantageous for shoot elongation. Callus induction occurred with TDZ, while control or IAA-only conditions exhibited shoot and root growth. Cytokinin addition led to callus formation and increased weight. Assessing survival and soil adaptation during in vitro hop seedling acclimatization involved maintaining near 100% humidity initially, gradually reducing it over three weeks. When transferred outdoors, 9 out of 10 seedlings acclimated successfully, confirming a 90% acclimatization rate.

Keywords
Acclimatization
BAP
Humulus lupulus L.
Regeneration
TDZ
Tissue culture
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 37
  • No :4
  • Pages :431-438
  • Received Date : 2024-03-19
  • Revised Date : 2024-06-20
  • Accepted Date : 2024-06-20
  • DOI :https://doi.org/10.7732/kjpr.2024.37.4.431
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