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2025 Vol.38, Issue 4 Preview Page

Research Article

Optimization of Extraction Conditions for Polyphenol Compound from Persicaria chinensis Leaf Using Response Surface Methodology

반응표면분석법을 이용한 덩굴모밀 잎의 폴리페놀 화합물 추출 조건 최적화

Daeho Choi1
,
Eun-Suk Jung1
,
Jungmok Kang1
,
Yong-Woo Park1
최 대호1
,
정 은숙1
,
강 정목1
,
박 용우1
1Researcher, Forest Bio Center, Chungcheongbuk-do Forest Environment Research Center, Okcheon 29061, Korea
1충북산림환경연구소 산림바이오센터, 연구원
*Corresponding Author
1 August 2025. pp. 349-358
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Abstract

This study was conducted using response surface analysis to confirm the optimal extraction conditions for efficient supply of polyphenol compound, which are indicator components of Persicaria chinensis, which is being developed as a resource plant. An experiment was performed on 17 sections using hot air dried leaves of P. chinensis by setting three independent variables (ethanol concentration, extraction time, extraction temperature) and the contents of neochlorogenic acid, corilagin, geraniin and ellagic acid were measured. The optimal extraction conditions for neochlorogenic acid were 19.2% ethanol concentration, 27.1 h extraction time, and 75°C extraction temperature. The expected value was 5.90 ㎎/g, and the experimental value was 5.82±0.25 ㎎/g. The optimal extraction conditions for geraniin were 41.5% ethanol concentration, 16.6 h extraction time, and 51.4°C extraction temperature. The expected value was 5.84 ㎎/g, and the experimental value was 5.95±0.44 ㎎/g. The optimal extraction conditions for ellagic acid were 80% ethanol concentration, 30 h extraction time, and 75°C extraction temperature. The expected value was 5.67 ㎎/g, and the experimental value was 7.45±1.25 ㎎/g. It was confirmed that neochlorogenic acid, geraniin and ellagic acid could be efficiently secured through optimal extraction conditions derived by response surface analysis.

Keywords
Extraction standardization
Marker compound
RSM
Secondary metabolite
References
1

Azwanida, N.N. 2015. A review on the extraction methods use in medicinal plants, principle, strength and limitation. Med. Aromat. Plants 4(3):196.

2

Baharuddin, N.S., M.A.M. Roslan, M.A.M. Bawzer, A.M. Azzeme, Z.A. Rahman, M.E. Khayat, N.A.A. Rahman, and Z.M. Sobri. 2021. Response surface optimization of extraction conditions and in vitro antioxidant and antidiabetic evaluation of an under-valued medicinal weed. Mimosa pudica. Plants 10(8):1692.

10.3390/plants1008169234451737PMC8399142
3

Breig, S.J.M., and K.J.K. Luti. 2021. Response surface methodology: A review on its applications and challenges in microbial cultures. Mater. Today: Proc. 42(5):2277-2284.

10.1016/j.matpr.2020.12.316
4

Chen, W., X.M. Shen, L. Ma, R. Chen, Q. Yuan, Y.F. Zheng, C.Y. Li, and G.P. Peng. 2020. Phenolic compounds from Polygonum chinense induce growth inhibition and apoptosis of cervical cancer SiHa cells. BioMed Res. Int. 2020(1): 8868508.

10.1155/2020/886850833381593PMC7762659
5

Cho, J.H., H.D. Jo, Y.E. Choi, S.H. Park, J.M. Yang, C.W. Jeong, D.H. Choi, E.S. Jung, Y.W. Park, and J.M. Kang. 2024. A composition for alleviating immune hypersensitivity comprising Persicaria chinensis extract. Korea Patent. 10-2024-0124042 (in Korean).

6

Choi, D., J. Kang, E.S. Jung, and Y.W. Park. 2024. Optimization of extraction conditions for verbascoside and rutin from Abeliophyllum distichum Nakai. leaf using response surface methodology. Korean J. Plant Res. 37(5):448-454 (in Korean).

7

Choi, D., J. Kang, E.S. Jung, and Y.W. Park. 2025. Polyphenol profiling and comparison of useful component content according to extraction solvents and plant part of Persicaria chinensis. Korean J. Plant Res. 38(1):18-25 (in Korean).

8

Choi, G.Y., Y.S. Han, K.H. Sim, and M.H. Kim. 2023. Phenolic compounds, antioxidant capacity, and α-amylase and α- glucosidase inhibitory activity of ethanol extracts of perilla seed meal. Food Sci. Nutr. 11(8):4596-4606.

10.1002/fsn3.341937576065PMC10420855
9

Hossen, M.J., K.S. Baek, E. Kim, W.S. Yang, D. Jeong, J.H. Kim, D.H. Kweon, D.H. Yoon, T.W. Kim, J.H. Kim, and J.Y. Cho. 2015. In vivo and in vitro anti-inflammatory activities of Persicaria chinensis methanolic extract targeting Src/Syk/NF-κB. J. Ethnopharmacol. 159:9-16.

10.1016/j.jep.2014.10.064
10

Kim, H.Y., and W.S. Lee. 2012. 3-Level response surface design by using expanded spherical experimental region. Korean J. Appl. Stat. 25(1):215-223 (in Korean).

10.5351/KJAS.2012.25.1.215
11

Kim, J.S., H.W. Jang, Y.S. Cho, and H.Y. Kim. 2023. Optimization of drying conditions for Chamchwi (Aster scaber Thunb.) using the response surface methodology with a central composition design. J. Korean Soc. Food Sci. Nutr. 52(8):844-855 (in Korean).

10.3746/jkfn.2023.52.8.844
12

Korea National Arboretum. 2021. The National Red List of Vascular Plants in Korea. Designpost, Pocheon, Gyeonggi, Korea (in Korean).

13

Kurita, S., T. Kashiwagi, T. Ebisu, T. shimamura, and H. Ukeda. 2016. Identification of neochlorogenic acid as the predominant antioxidant in Polygonum cuspidatum leaves. Ital. J. Food Sci. 28(1):25-31.

14

Lai, S.M., D. Sudhahar, and K. Anandarajagopal. 2012. Evaluation of antibacterial and antifungal activities of Persicaria chinensis leaves. Int. J. Pharm. Sci. Res. 3(8):2825-2830.

15

Li, X., Y. Deng, Z. Zheng, W. Huang, L. Chen, Q. Tong, and Y. Ming. 2018. Corilagin, a promising medicinal herbal agent. Biomed. Pharmacother. 99:43-50.

10.1016/j.biopha.2018.01.030
16

Lim, W.S., S.Y. Ha, J.Y. Jung, H.C. Kim, and J.K. Yang. 2024. Optimization of DPPH radical scavenging activity by response surface methodology in the ultrasonic-assisted extraction of Lespedeza bicolor Turcz. J. Agri. Life Sci. 58(2):65-80.

10.14397/jals.2024.58.2.65
17

Luo, J., A. Wei, H. Zhu, F. Xie, G. Huang, P. Huang, W. Yang, L. Deng, S. Lin, and H. Qin. 2024. Optimization of extraction process and content determination of total flavonoids from Polygonum chinense L. produced in Guangxi. Hubei Agri. Sci. 63(1):164-168.

18

Myers, R.H. 1971. Response Surface Methodology. Allyn and Bacon, Inc., Boston, MA (USA). pp. 126-256.

19

On-Nom, N., S. Thangsiri, W. Inthachat, P. Temviriyanukul, Y. Sahasakul, A. Aursalung, C. Chupeerach, and U. Suttisansanee. 2024. Optimized conditions for the extraction of phenolic compounds from Aeginetia indica L. and its potential biological applications. Molecules 29(5):1050.

10.3390/molecules2905105038474563PMC10935255
20

Palanikumar, K. 2021. Response Surface Methodology in Engineering Science. IntechOpen Limited, London, United Kingdom.

10.5772/intechopen.100484
21

Park, S.H. 2022. Modern Design of Experiment. Minyoungsa, Seoul, Korea (in Korean).

22

Park, S.J., S.W. Moon, J. Lee, E.J. Kim, and B.S. Kang. 2011. Optimization of roasting conditions for coffee beans by response surface methodology. Korean J Food Preserv. 18:178-183 (in Korean).

10.11002/kjfp.2011.18.2.178
23

Perera, A., S.H. Ton, and U.D. Palanisamy. 2015. Perspectives on geraniin, a multifunctional natural bioactive compound. Tr. Food Sci. Technol. 44(2):243-257.

10.1016/j.tifs.2015.04.010
24

Rheem, S.S., and I.S. Rheem. 2012. The model-fit proportion and the coefficient of pure error variation, the measures to supplement the lack-of-fit test in response surface analysis. J. Korean Data Anal. Soc. 14(6):2989-2994 (in Korean).

25

Ríos, J.L., R.M. Giner, M. Marín, and M.C. Recio. 2018. A pharmacological update of ellagic acid. Planta Med. 84(15): 1068-1093.

10.1055/a-0633-9492
26

Rohilla, S., and C.L. Mahanta. 2021. Optimization of extraction conditions for ultrasound-assisted extraction of phenolic compounds from tamarillo fruit (Solanum betaceum) using response surface methodology. J. Food Meas. Charact. 15:1763-1773.

10.1007/s11694-020-00751-3
27

Tapsell, L.C., I. Hemphill, L. Cobiac, D.R. Sullivan, M. Fenech, C.S. Patch, S. Roodenrys, J.B. Keogh, P.M. Clifton, and P.G. Williams. 2006. Health benefits of herbs and spices: the past, the present, the future. Medi. J. Aust. 185:S4-S24.

10.5694/j.1326-5377.2006.tb00548.x
28

Wu, J., F. Yang, L. Guo, and Z. Sheng. 2024. Modeling and optimization of ellagic acid from chebulae fructus using response surface methodology coupled with artificial neural network. Molecules 29(16):3953.

10.3390/molecules2916395339203031PMC11357226
29

Yang, X., Y. Li, Q. Shao, Z. Li, Z. Chen, Y. Wang, Y. Zhou, and R. Chen. 2024. Screening, fingerprinting, and identification of phenolic antioxidants in Persicaria chinensis (L.) H. Gross by liquid chromatography – electrochemical detection and liquid chromatography – tandem mass spectrometry. J. Chromatogr. B. 1250:124387.

10.1016/j.jchromb.2024.124387
30

Yang, Y.C., J. Li, Y.G. Zu, M. Luo, N. Wu, and X.L. Liu. 2010. Optimisation of microwave-assisted enzymatic extraction of corilagin and geraniin from Geranium sibiricum Linne and evaluation of antioxidant activity. Food Chemistry 122(1):373-380.

10.1016/j.foodchem.2010.02.061
31

Zhang, L.L., M. Xu, Y.M. Wang, D.M. Wu, and J.H. Chen. 2010. Optimizing ultrasonic ellagic acid extraction conditions from infructescence of Platycarya strobilacea using response surface methodology. Molecules 15(11):7923-7932.

10.3390/molecules1511792321060299PMC6259309
32

Zhu, T., H.J. Heo, and K.H. Row. 2010. Optimization of crude polysaccharides extraction from Hizikia fusiformis using response surface methodology. Carbohydr Polym. 82:106-110.

10.1016/j.carbpol.2010.04.029
Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 38
  • No :4
  • Pages :349-358
  • Received Date : 2025-02-08
  • Revised Date : 2025-04-06
  • Accepted Date : 2025-04-21
  • DOI :https://doi.org/10.7732/kjpr.2025.38.4.349
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