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2025 Vol.38, Issue 4 Preview Page

Research Article

Antioxidant and Photoprotective Effects of Pinus rigida Cone Extract in UVB-Irradiated Hs68 Human Dermal Fibroblasts

리기다(Pinus rigida) 솔방울 추출물의 항산화 활성 및 UVB 유발 Hs68 세포 손상 억제 효과

Yong-Shin Kim12
,
Cheol Kyu Lee2
,
Jae Ho Park23
김 용신12
,
이 철규2
,
박 재호23
1Professor, Department of Beauty Medical, Jungwon University, Goesan 28027, Korea
2Graduate Student, Department of Biohealth Industry, Jungwon University, Goesan 28027, Korea
3Professor, Department of Bioindustry, Jungwon University, Goesan 28027, Korea
1중원대학교 뷰티메디컬학과, 교수
2중원대학교 대학원 바이오헬스산업학과, 대학원생
3중원대학교 바이오산업학과, 교수
*Corresponding Author
1 August 2025. pp. 342-348
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Abstract

This study evaluated the antioxidant and photoprotective effects of Pinus rigida cone extract (ECRT) in UVB-irradiated Hs68 human dermal fibroblasts. Antioxidant capacity was measured using DPPH and ABTS radical scavenging assays, showing a dose-dependent increase with activity comparable to L-ascorbic acid at concentrations above 40 μg/mL. Cytotoxicity assays confirmed that ECRT was non-toxic to Hs68 cells at 25–400 μg/mL. Upon UVB exposure, DNA damage markers γ-H2AX and p53 were significantly elevated, but ECRT treatment reduced their expression in a concentration-dependent manner, demonstrating DNA protection. Additionally, ECRT inhibited UVB-induced expression of matrix metalloproteinases MMP-1, MMP-3, and MMP-9, which are key enzymes involved in skin aging and wrinkle formation, with notable suppression at 100–200 μg/mL. These findings indicate that ECRT not only scavenges free radicals but also protects dermal fibroblasts from UVB-induced oxidative damage and photoaging. Consequently, Pinus rigida cone extract shows promise as a safe, effective, and eco-friendly ingredient for skin photoprotection and anti-aging applications.

Keywords
Antioxidant
Anti-wrinkle
DNA damage
Photoprotection
Pinus rigida cone extract
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 38
  • No :4
  • Pages :342-348
  • Received Date : 2025-06-09
  • Accepted Date : 2025-06-12
  • DOI :https://doi.org/10.7732/kjpr.2025.38.4.342
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