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2021 Vol.34, Issue 1 Preview Page

Research Article

Comparison of Growth Characterisitics, Asiaticoside Content and Antioxidant Activities of Centella asiatica (L.) Urb.

병풀의 생육 특성, Asiaticoside 함량 및 항산화 활성 비교

Jang Nam Choi1†
,
Myeong Won Oh1†
,
Hee Jung Lee2
,
Jeong Hoon Lee3
,
Jin Tae Jeong3
,
Yun Ji Lee3
,
Jae Ki Chang4
,
Chun Geon Park4†
최 장남1†
,
오 명원1†
,
이 희정2
,
이 정훈3
,
정 진태3
,
이 윤지3
,
장 재기4
,
박 춘근4†
1Post-doc, Herbal Crop Research Division, Herbal Crop Research Division, National Institute of Horticultural and Herbal Science, RDA, Eumseong 27709, Korea
2Postgraduate Student, Department of Food Science and Technology, Chungbuk National University, Cheongju 28644, Korea
3Researcher, Herbal Crop Research Division, National Institute of Horticultural and Herbal Science, RDA, Eumseong 27709, Korea
4Senior Researcher, Herbal Crop Research Division, National Institute of Horticultural and Herbal Science, RDA, Eumseong 27709, Korea
1농촌진흥청 국립원예특작과학원 약용작물과, 박사후연구원
2충북대학교 식품공학과, 대학원생
3농촌진흥청 국립원예특작과학원 약용작물과, 농업연구사
4농촌진흥청 국립원예특작과학원 약용작물과, 농업연구관
†These authors contributed equally to this work.
*pcg@korea.kr
1 February 2021. pp. 44-51
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Abstract

Centella asiatica (L.) Urban is a medicinal plant containing madecassic acid, triterpenoids and asiaticoside, and has a wound healing effect. However, since it grows only in some regions of Korea, it is necessary to breed stable varieties. In this study, it were analyzed for the growth characteristics, asiaticoside, anxiodant components and 2,2-diphenyl-1picryl hydrazyl (DPPH) radical scavening activity of C. asiatica collected from domestic natural habitat (Hapcheon, Gyeongnam). In the growth characteristics of each resource, CA9 and CA10 were superior in characteristics of aerial part and in dry weight. In asiaticoside content analysis, CA5 and CA3 were high with 53.96 ㎎/g and 52.40 ㎎/g, respectively. CA12 showed the possibility of development because the resource showed high in total flavonoid (7.3 ㎎/g), total polyphenol (10.64 ㎎/g) and DPPH activity (11.96 ㎎ AAE/g) among other resources. However, it is necessary to select a resource suitable for each characteristic of C. asiatica and to study charateristics through additional resources collection since the excellent growth characteristics of the collected resources do not necessarily coincide with the content and antioxidant activities.

Keywords
Asiaticoside
Antioxidant activity
Centella asiatica
Total flavonoid
Total polyphenol
References
1
Abe, N., T. Murata and A. Hirota. 1998. Novel DPPH radical scavengers, bisorbicillinol and demethyltrichodimerol, from a fungus. Biosic. Biotech. and Bioch. 62(4):661-666. 10.1271/bbb.62.66127392553
2
Baek, Y.W. 1997. Micropropagation of Centella asiatica (L) Urban by in vitro cultures and production of triterpene glycosides. Department of Biology, Ph.D. Thesis, Chonnam National University, Korea. p. 13 (in Korean).
3
Brinkhaus, B., M. Linder, D. Schuppan and E.G. Hahn. 2000. Chemical, pharmacological and clinical profile of the east Asian medical plant Centella asiatica. Phytomedicine 7(5):427-448. 10.1016/S0944-7113(00)80065-3
4
Chasseaud, L.P., B.J. Fry, D.R. Hswkins, J.D. Lewis, I.P. Sword, T. Taylor and D.E. Hathway. 1971. The metabolism of asiatic acid, madecassic acid and asiaticoside in the rat. Arzneimittel-Forsch. 21(9):1379-1384.
5
Cho, C.H., S.Y. Kim and I.S. An. 2014. The improving effect of Centella asiatica extracts on erythema on scalp of aged 20-50's women. Kor. J. Aesthet. Cosmetol. 12(6):921-927 (in Korean).
6
Choi, J.N., H.J. Lee, Y.J. Lee, J.T. Jeong, J.H. Lee, J.K. Chang and C.G. Park. 2020. Growth characteristics and asiaticoside content, and antioxidant activities in Centella asiatica by cultivation and irrigation methods. Korean J. Med. Crop Sci. 28(4):254-259 (in Korean). 10.7783/KJMCS.2020.28.4.254
7
Davis, W.B. 1947. Determination of flavanones in citrus fruits. Anal. Chem. 19(7):476-478. 10.1021/ac60007a016
8
Folin, O. and W. Denis. 1912. Tyrosine in proteins as determined by a new colorimetric method. J. Biol. Chem. 12(2):245-251. 10.1016/S0021-9258(18)88698-7
9
Gohil, K.J., J.A. Patel and A.K. Gajjar. 2010. Pharmacological review on Centella asiatica: A potential herbal cure-all. Indian J. Pharm. Sci. 72(5):546. 10.4103/0250-474X.7851921694984PMC3116297
10
Griesbach, R.J., P. Semeniuk, S.M. Roh and R.H. Lawson. 1988. Tissue culture in the improvement of Eustoma. Hortic. Sci. 23:790-791.
11
Ha, J.H., M.C. Kwon, S.S. Kim, M.H. Jeong, B. Hwang and H.Y. Lee. 2010. Enhancement of skin-whitening and UV-protective effects of Centella asiatical L. Urban by Utrasosonification process. Korean J. Med. Crop Sci. 18(2):79-85 (in Korean).
12
Hausen, B.M. 1993. Centella asiatica (Indian pennywort), an effective therapeutic but a weak sensitizer. Contact Derm. 29(4):175-179. 10.1111/j.1600-0536.1993.tb03532.x8281778
13
Jayashree, G., G.K. Muraleedhara, S. Sudarslal and V. Jacob. 2003. Anti-oxidant activity of Centell asiatica on lymphoma-bearing mice. Fitoterapia 74(5):431-434. 10.1016/S0367-326X(03)00121-7
14
Kil, Y.S., S.M. Sin, D.Y. Lee, W.M. Jeong, K.J. Yang, S.W. Lee, Y.H. Kim and Y.M. Goo. 2018. Analysis of triterpene glycoside levels and antioxidant activity in the different shoot tissues of Centella asiatica (L.) Urban. J. Life Sci. 28(8):917-922 (in Korean).
15
Kim, J., M.J. Kim and K.Y. Lee. 2013. Wound healing effect of curcumin gel for transdermal delivery. Polymer (Korea) 37(3):387-392 (in Korean). 10.7317/pk.2013.37.3.387
16
Kim, O.T., M.Y. Kim, Y.J. Park, M.H. Hong, J.C. Ahn, M.H. Oh and B. Hwang. 2002. Production of triterpene glycosides from whole plant cultures of Centell asiatica (L.) Urban. Korean J. Plant Biotechnol. 29(4):281-285 (in Korean). 10.5010/JPB.2002.29.4.281
17
Kim, S.J., H.I. Kim, Y.Y. Hur, D.J. Im, D.H. Lee, S.J. Park, S.M. Jung and K.H. Chng. 2019. Anthocyanin and polyphenol analysis and antioxidant activity of small fruit and berries in Korea. Korean J. Plant Res. 32(5):407-414 (in Korean).
18
Kim, Y.J. 2010. Effects of Centell asiatica extracts on anti-oxidant and collagen synthesis according to extraction conditions. J. Kor. Soc. Cosm. 16(3):834-839 (in Korean).
19
Koo, Y.M., Y.S. Kil, S.M. Sin, D.Y. Lee, W.M. Jeong, K.H. Ko, K.J. Yang, Y.H. Kim and S.W. Lee. 2018. Analysis of antibacterial, anti-inflammatory, and skin-whitening effect of Centella asiatica (L.) Urban. J. Plant Biotechnol. 45(2):117-124 (in Korean). 10.5010/JPB.2018.45.2.117
20
Korea Forest Service. 2003. Korean Plant Names Index (accessed 2003. 11. 30). http://www.nature.go.kr.
21
Mook-Jung, I.H., J.E. Shin, S.H. Yun, K. Huh, J.Y. Koh, H.K. Park, S.S. Jew and M.W. Jung. 1999. Protective effects of asiaticoside derivatives against beta-amyloid neurotoxicity. J. Neurosci. Res. 58(3):417-425. 10.1002/(SICI)1097-4547(19991101)58:3<417::AID-JNR7>3.0.CO;2-G
22
Pittella, F., R.C. Dutra, D.D. Junior, M.T. Lopes and N.R. Barbosa. 2009. Antioxidant and cytotoxic activities of Centella asiatica (L.) Urb. Int. J. Mol. Sci. 10(9):3713-3721. 10.3390/ijms1009371319865514PMC2769141
23
Sritongkul, J., V. Srilaong, A. Uthairatanakij, S. Kanlayanarat and P. Chalermglin. 2009. Effect of light intensity on chemical composition of asiatic pennywort (Centella asiatica L. Urban). Acta Hortic. 837:87-93. 10.17660/ActaHortic.2009.837.9
24
World Health Organization (WHO) 1999. WHO Monographs on Selected Medicinal Plants (Vol. 1). Geneva, Switzerland. pp. 77-85.
25
Zhang, M., N.S. Hettiarachchy, R. Horax, A. Kannan, P. Apputhury, A. Muhundan and C.R. Mallangi. 2011. Phytochemicals, antioxidant and antimicrobial activity of Hibiscus sabdariffa, Centella asiatica, Moringa oleifera and Murraya koenigii leaves. J. Med. Plant Res. 5(30):6672-6680. 10.5897/JMPR11.621
Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 34
  • No :1
  • Pages :44-51
  • Received Date : 2020-08-19
  • Revised Date : 2020-10-19
  • Accepted Date : 2020-10-22
  • DOI :https://doi.org/10.7732/kjpr.2021.34.1.044
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