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2023 Vol.36, Issue 3 Preview Page
Suppression of Migration and Invasion by Alnus hirsuta in Human Hepatocellular Carcinoma Cells
Bo-Ram Kim1
,
Su Hui Seong1
,
Tae-Su Kim3
,
Jin-Ho Kim1
,
Chan Seo1
,
Ha-Nul Lee1
,
Sua Im2
,
Jung Eun Kim2
,
Ji Min Jung2
,
Jung Up Park1
,
Kyung-Min Choi5
,
Jin-Woo Jeong4*
1Associate Researcher, Honam National Institute of Biological Resources, Mokpo 587262, Korea
2Researcher, Honam National Institute of Biological Resources, Mokpo 587262, Korea
3Senior Researcher, Honam National Institute of Biological Resources, Mokpo 587262, Korea
4General Manager, Honam National Institute of Biological Resources, Mokpo 587262, Korea
5Director, Honam National Institute of Biological Resources, Mokpo 587262, Korea
*Corresponding Author
1 June 2023. pp. 207-218
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Abstract

Hepatocellular carcinoma (HCC) has a poor prognosis and high metastasis and recurrence rates. Although extracts of Alnus hirsuta (Turcz. ex Spach) Rupr. (AH) have been demonstrated to possess potential anti-inflammatory and anti-cancer activities, the underlying mechanism of AH in HCC treatment remains to be elucidated. We investigated the effects and potential mechanisms of AH on migration and invasion of Hep3B cells. Within the non-cytotoxic concentration range, AH significantly inhibited motility and invasiveness of Hep3B cells in a concentration-dependent manner. Inhibitory effects of AH on cell invasiveness are associated with tightening of tight junctions (TJs), as demonstrated by an increase in transepithelial electrical resistance. Immunoblotting indicated that AH decreased levels of claudins, which form major components of TJs and play key roles in the control and selectivity of paracellular transport. Furthermore, AH inhibited the expression and activity of matrix metalloproteinase (MMP)-2 and MMP-9 and simultaneously increased the levels of tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2. These effects were related to inactivation of the phosphoinositide 3-kinase (PI3K)/AKT pathway in Hep3B cells. Therefore, AH inhibits migration and invasion of Hep3B cells by inhibiting the activity of MMPs and tightening TJs through suppression of claudin expression, possibly by suppressing the PI3K/AKT signaling pathway.

Keywords
Alnus hirsuta (Turcz. ex Spach) Rupr.
Hepatocellular carcinoma Hep3B cells
Invasion
Migration
PI3K/AKT
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 36
  • No :3
  • Pages :207-218
  • Received Date : 2023-05-04
  • Revised Date : 2023-05-18
  • Accepted Date : 2023-05-19
  • DOI :https://doi.org/10.7732/kjpr.2023.36.3.207
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