Hepatoprotective Effect of Alnus japonica and Portulaca oleracea Complex on Alcohol-induced Liver Injury Mice Models by Anti-oxidation Activity

Dong ki Hong; Soodong Park; Jooyun Kim; Jaejung Shim; Junglyoul Lee

doi:10.7732/kjpr.2023.36.3.198

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2023 Vol.36, Issue 3 Preview Page Next Page

Hepatoprotective Effect of Alnus japonica and Portulaca oleracea Complex on Alcohol-induced Liver Injury Mice Models by Anti-oxidation Activity

1 June 2023. pp. 198-206

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Abstract

The effectiveness of the extracts of Alnus japonica and Portulaca oleracea, which are effective in improving alcohol-induced liver damage, was confirmed using acute and chronic alcoholic liver injury animal models. In the acute alcoholic liver injury model, dieting Alnus japonica and Portulaca oleracea complex (ALPOC) at a dose of 50 ㎎/㎏ showed no significant change in liver or body weight, while measured plasma ALT activity to be deficient (28.12 U/ml) compared to the alcohol intake group (42.5 U/ml), and confirmed that restored it to an average level. It showed an improvement of 34.9% compared to the alcohol intake group. AST activity confirmed that it showed a very effective liver protection activity by showing a gain of 12.6%. The chronic alcoholic liver damage animal model demonstrated that ALT showed an improvement effect of 25%, and AST showed an effect similar to that of the positive control group, Hovenia extract. In addition, through H&E staining analysis, observed that the ALPOC improved the necrosis and bleeding of the liver. And the ALPOC group showed intense antioxidant activity of 127% or more compared to the alcohol intake group, and this was confirmed to have a very high activity, which is more than 20% higher than that of the hovenia fruit extract.

Keywords

Alcoholic disease

Alnus japonica

Antioxidant

Portulaca oleracea

Hepatoprotective complex

References

Albano, E. 2002. Free radical mechanisms in immune reactions associated with alcoholic liver disease. Free Radic. Biol. Med. 32:110-114. 10.1016/S0891-5849(01)00773-011796198

An, S.W., Y.G. Kim, M.H. Kim, B.I. Lee, S.H. Lee, H.I. Kwon, B. Hwang and H.Y. Lee. 1999. Comparison of hepatic detoxification activity and reducing serum alcohol concentration of Hovenia dulsis THUNB and Alnus japonica steud. Korean J. Medicinal Crop Sci. 7:263-268.

Chayanupatkul, M. and S. Liangpunsakul. 2014. Alcoholic hepatitis: a comprehensive review of pathogenesis and treatment. World J Gastroenterol. 20(20):6279-6286. 10.3748/wjg.v20.i20.627924876748PMC4033465

Farkhondeh, T. and S. Samarghandian. 2019. The therapeutic effects of Portulaca oleracea L. in hepatogastric disorders. Gastroenterol. Hepatol. 42:127-132. 10.1016/j.gastrohep.2018.07.01630327154

Holmström, K.M. and T. Finkel. 2014. Cellular mechanisms and physiological consequences of redox-dependent signaling. Nat Rev Mol Cell Biol. 15:411-421. 10.1038/nrm380124854789

Kim, O.K. 2003. Protective effects of Alnus japonica steude on hepatic injury induced by carbon tetrachloride in rats. Kor. J. Pharmacogn. 34(2):172-178.

Kim, K.A. 2009. Current status of liver diseases in Korea: toxic and alcoholic liver diseases. Korean J. Hepatol. 15:29-33. 10.3350/kjhep.2009.15.S6.S2920037277

Kim, M.J., H.J. Yang, H.Y. Lee, D.Y. Shin, Y.H. Lee, Y.G. Kang, T.S. Kim, S.T. Lee and K.H. Park. 2020. Improving effects of Brassica oleracea L. var. italica sprout extract on alcoholic liver dysfunction. Korean J. Plant Res. 33(3):163-169.

Kim, S. T., J.D. Kim, S.H. Ahn, G.S. Ahn, Y.I. Lee and Y.S. Jeong. 2004. Hepatoprotective and antioxidant effects of Alnus japonica extract on acetaminophen-induced hepatotoxicity in rats. Phytother. Res. 18:971-975. 10.1002/ptr.154015742342

Lee, M.W., J.H. Kim, D.W. Jeong, K.H. Ahn, S.H. Toh and Y.J. Surh. 2000a. Inhibition of cyclooxygenase-2 expression by diarylheptanoids from the bark of Alnus hirsuta var. sibrica. Biol. Pharm. Bull. 23:517-518. 10.1248/bpb.23.51710784440

Lee, U.E. and S.L. Friedman. 2011. Mechanisms of hepatic fibrogenesis. Best Pract. Res. Clin. Gastroenterol. 25:195-206. 10.1016/j.bpg.2011.02.00521497738PMC3079877

Lee, Y.A., K.H. Kim, J.S. Kim, S.M. Cho, S.W. Kim and M.W. Lee. 2000b. Antioxidative effects of diarylheptanoids from Alnus hirsuta. Yakhak Hoeji 44:193-196.

Lieber, C.S. 2004. Milestones in liver disease. J. Hepatol. 40:198-202. 10.1016/j.jhep.2003.12.00514739088

Lim, H.K., H.S. Kim, H.S. Choi, and J.W. Choi. 1999. Protective and therapeutic effects of Malloti cortex extract on carbon tetrachloride and galactosamine-induced hepatotoxicity in rats. J. Appl. Pharmacol. 7:35-43.

Lu, S.C. and J.M. Mato. 2008. S-Adenosylmethionine in cell growth, apoptosis, and liver cancer. J. Gastroenterol. Hepatol. 23:73-77. 10.1111/j.1440-1746.2007.05289.x18336669PMC2408691

Plaa, G.L. and M. Charbonneau. 1994. Detection and evaluation of chemically induced liver injury. In Hayes, A.W. (ed.), Principles and Methods of Toxicology. Raven press, New York, NY (USA). pp. 839-870.

Qiao, J.Y., H.W. Li, F.G. Liu, Y.C. Li, S. Tian, L.H Cao, K. Hu, X.X. Wu and M.S. Miao. 2019. Effects of Portulaca oleracea extract on acute alcoholic liver injury of rats. Molecules 24(16): 2887. 10.3390/molecules2416288731398934PMC6720614

Rasineni, K. and C.A. Casey. 2012. Molecular mechanism of alcoholic fatty liver. Indian J. Pharmacol. 44:299-303. 10.4103/0253-7613.9629722701235PMC3371448

Tung, N.H., S.K. Kim, J.C. Ra, Y.Z. Zhao, D.H. Sohn and Y.H. Kim. 2010. Antioxidative and hepatoprotective diarylheptanoids from the bark of Alnus japonica. Planta Med. 76:626-629. 10.1055/s-0029-124059519918716

Uddin, K., A.S. Juraimi, S. Hossain, A.U. Nahar, E. Ali and M. M. Rahman. 2014. Purslane weed (Portulaca oleracea): a prospective plant source of nutrition, omega-3 fatty acid, and antioxidant attributes. Sci World J. 2014, 951019. 10.1155/2014/95101924683365PMC3934766

Wheeler, M.D., M. Nakagami, B.U. Bradford, T. Uesugi, R.P. Mason and H.D. Connor. 2001. Overexpression of manganese superoxide dismutase prevents alcohol-induced liver injury in the rat. J. Biol. Chem. 276:36664-36672. 10.1074/jbc.M10535220011477087

World Health Organization (WHO). 2011. Global Status Report on Alcohol and Health 2011. Alcohol, Drugs, and Addictive Behaviors. World Health Organization.

World Health Organization (WHO). 2014. Global Status Report on Noncommunicable Diseases-2014. World Health Organization.

Yin, H., M.X. Hu, J.M. Liang, X.L. Ajmo and R. Bataller. 2014. Deletion of SIRT1 from hepatocytes in mice disrupts lipin-1 signaling and aggravates alcoholic fatty liver. Gastroenterology 146(3):801-811. 10.1053/j.gastro.2013.11.00824262277PMC3943758

Information

Publisher :The Plant Resources Society of Korea
Publisher(Ko) :한국자원식물학회
Journal Title :Korean Journal of Plant Resources
Journal Title(Ko) :한국자원식물학회지
Volume : 36
No :3
Pages :198-206
Received Date : 2023-05-04
Revised Date : 2023-05-16
Accepted Date : 2023-05-17
DOI :https://doi.org/10.7732/kjpr.2023.36.3.198

Korean Journal of Plant Resources ISSN:1226-3591(Print) 2287-8203(Online) 한국자원식물학회지

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