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2020 Vol.33, Issue 4 Preview Page

Research Article

Identification and Characterization of Fibrinolytic Compound from Cornus officinalis S. et Z

산수유(Cornus officinalis)로부터 혈전용해물질의 확인 및 특성 연구

Jun-Ho Kim1*
김 준호1*
1Professor, Department of Fine Chemistry and New Materials, Sangji University, Wonju 26339, Korea
1상지대학교 정밀화학신소재학과, 교수
* Corresponding Author
1 August 2020. pp. 237-244
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Abstract

The objective of this study was to identify and characterize fibrinolytic compound from Cornus officinalis. Cornus officinalis. Hot water extract was fractionated into hexane, chloroform, ethyl acetate, butanol, and water fractions. Assays for fibrinolytic activity indicated that only the ethyl acetate fraction had significant efficacy at 1.36 plasmin units/mL. Isolation of fibrinolytic compound was carried out on Amberlite IRA-400, Sephadex LH-20 and active charcoal column chromatography. HPLC analysis of the purified fibrinolytic compound showed retention time (RT) same as authentic malic acid. LC / MS / MS in negative mode showed the same peak at m/z 133, confirming that the purified compound was malic acid with a molecular weight 134 Da. The compound showed fibrinolytic activity of 0.69 plasmin units/mL, 14.62% of thrombin inhibitory activity, 6.42% of antioxidative activity, and 17.28% of α-glucosidase inhibitory activity. The purified compound hydrolyzed γ subunits of human fibrinogen. In conclusion, malic acid isolated from Cornus officinalis might have potential to be developed as ingredient for biofunctional foods to prevent cardiovascular diseases.

Keywords
Cornus officinalis
Fibrinolytic activity
HPLC
LC/MS/MS
Malic acid
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 33
  • No :4
  • Pages :237-244
  • Received Date : 2020-02-25
  • Revised Date : 2020-04-07
  • Accepted Date : 2020-04-16
  • DOI :https://doi.org/10.7732/kjpr.2020.33.4.237
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