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2021 Vol.34, Issue 1 Preview Page

Research Article

Anti-inflammatory Effects of Hemistepta lyrata Bunge in LPS-stimulated RAW 264.7 Cells through Regulation of MAPK Signaling Pathway

LPS로 유도된 RAW 264.7 대식세포의 염증반응에서 MAPK 신호경로 조절을 통한 지칭개 에탄올 추출물의 항염증 효과

Chul Hwan Kim†
,
Young-Kyung Lee
,
Jin-Woo Jeong
,
Buyng Su Hwang
,
Yong Tae Jeong
,
Yong Taek Oh
,
Pyo Yun Cho
,
Chang-Hee Kang††
김 철환†
,
이 영경
,
정 진우
,
황 병수
,
정 용태
,
오 영택
,
조 표연
,
강 창희††
Researcher, Nakdonggang National Institute of Biological Resources, Sangju-si 37242, Korea
†These authors contributed equally to this work.
*ckdgml3735@nnibr.re.kr
1 February 2021. pp. 23-30
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Abstract

Hemistepta lyrata Bunge (HL) has been used as a folk remedy to treat cancer, inflammation, bleeding, hemorrhoids and fever, and leaves and young shoots have been used as famine food. Nevertheless, the biological activities and underlying mechanisms of the anti-inflammatory effects remain unclear. In this study, it was undertaken to explore the functions of the aerial part of HL as a suppressor of inflammation by using RAW 264.7 cells. As immune response parameters, the productions of as nitric oxide (NO) and prostaglandin E2 (PGE2), cytokines such tumor necrotic factor (TNF)-α and interleukin (IL)-6 were evaluated. Although the release of TNF-α remained unchanged in HL-treated RAW 264.7 cells, the productions of NO, PGE2 and IL-6 were significantly increased at concentrations with no cytotoxicity. Furthermore, HL significantly attenuated the mitogen-activated protein kinases (MAPK) pathway including decreasing the phosphorylation of the extracellular signal-regulated kinase (ERK1/2) and p38 mitogen-activated protein kinases. Collectively, this study provides evidence that HL inhibits the production of major pro-inflammatory molecules in LPS-stimulated RAW 264.7 cells via suppression of ERK and P38 MAPK signaling pathways. These findings suggest that the beneficial therapeutic effects of HL may be attributed partly to its ability to modulate immune functions in macrophages.

Keywords
Cytokine
Hemistepta lyrata
Inflammation
MAPK
RAW 264.7 cells
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 34
  • No :1
  • Pages :23-30
  • Received Date : 2020-09-04
  • Revised Date : 2020-10-13
  • Accepted Date : 2020-10-21
  • DOI :https://doi.org/10.7732/kjpr.2021.34.1.023
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