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2023 Vol.36, Issue 3
Hibiscus syriacus Leaves Upregulate p62/SQSTM1 through TLR4/p38, JNK, and NF-κB/Nrf2 Signaling Pathway in RAW264.7 Cells
Seung Woo Im1†
,
Gwang Hun Park2†
,
Min Yeong Choi3
,
Hae-Yun Kwon4
,
Jin Boo Jeong5*
1Graduate Student, Department of Forest Sciences, Andong National University, Andong 36729, Korea
2Researcher, Forest Medicinal Resources Research Center, National Institute of Forest Science, Yeongju 36040, Korea
3Master’s Degree Researcher, Forest Medicinal Resources Research Center, National Institute of Forest Science, Yeongju 36040, Korea
4Senior Researcher, Forest Medicinal Resources Research Center, National Institute of Forest Science, Yeongju 36040, Korea
5Professor, Department of Forest Sciences, Andong National University, Andong 36729, Korea
*Corresponding Author
†Theses authors contributed equally to this work.
1 June 2023. pp. 191-197
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Abstract

Autophagy contributes to enhancing the immune system (innate and adaptive immune system) against foreign pathogens. Autophagy of macrophages is used as a major indicator for developing vaccine adjuvants to increase the adaptive immune response. In this study, HSL increased p62/SQSTM1 expression. Inhibition of TLR4, p38, JNK, and NF-κB blocked HSL-mediated increase of p62/SQSTM1. HSL activated p38, JNK, and NF-κB signaling, but HSL-mediated activation of p38, JNK, and NF-κB signaling was reversed by TLR4 inhibition. In addition, HSL increased Nrf2 expression, but HSL-mediated Nrf2 expression did not occur in the inhibition of TLR4, p38, JNK, and NF-κB. Taken together, it is believed that HSL-mediated autophagy may be dependent on activating Nrf2 expression via TLR4-dependent activation of p38, JNK, and NF-κB in macrophages.

Keywords
Autophagy
Hibiscus syriacus
Immunostimulation
Macrophages
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 36
  • No :3
  • Pages :191-197
  • Received Date : 2023-01-30
  • Revised Date : 2023-04-24
  • Accepted Date : 2023-05-02
  • DOI :https://doi.org/10.7732/kjpr.2023.36.3.191
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