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2023 Vol.36, Issue 5 Preview Page
Expression of Organogenesis-related Genes and Analysis of Genetic Stability by ISSR Markers of Regenerants Derived from the Process of in vitro Organogenesis in Japanese Blood Grass (Imperata cylindrica ‘Rubra’)

기내배양 홍띠 단계별 재분화체의 기관분화 관련 유전자 발현과 ISSR에 기반한 유전적 안정성 분석

Ye-Jin Lee1
,
In-Jin Kang1
,
Chang-Hyu Bae23*
이 예진1
,
강 인진1
,
배 창휴23*
1Graduate School Student, Department of Plant Production Sciences, Graduate School of Sunchon National University, Suncheon 57922, Korea
2Professor, Department of Plant Production Sciences, Graduate School of Sunchon National University, Suncheon 57922, Korea
3Professor, Department of Agricultural Life Sciences, Sunchon National University, Suncheon 57922, Korea
1순천대학교 대학원 식물생산과학부, 대학원생
2순천대학교 대학원 식물생산과학부, 교수
3순천대학교 농생명과학과 교수
*Corresponding Author
1 October 2023. pp. 496-507
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Abstract

The in vitro organogenesis is one of important issues in plant embryology, and somaclonal variations are existing in calli and/or regenerants induced from a process of the organogenesis with in vitro circumstances. In this study, expressions of organogenesis-related genes were evaluated and genetic stability of regenerants derived from the process of in vitro organogenesis were measured using ISSR markers in Imperata cylindrica ‘Rubra’, Poaceae. The expressions of organogenesis-related genes were detected all of regenerants at the process of the organogenesis. All ISSR markers produced with an average of 71 bands per in vitro-cultured regenerants, and the scorable bands were varied from two to eight with an average of 5.14 bands per a primer. The polymorphism rates of the in vitro regenerants were higher than that of mother plants (1.4%), showing 4.1% (pot-cultured regenerants), 4.3% (field-cultured regenerants), 4.2% (in vitro-cultured regenerants), 5.6% (calli with green shoots) and 1.4% (calli), respectively. The genetic similarity matrix (GSM) among all accessions ranged from 0.747 to 1.0 with a mean of 0.868. GSM of the regenerants showed differences (from 0.972 to 1.00) compared with that of mother plants (0.991). According to the clustering analysis, two independent groups were divided into; the one is mother plants and regenerants cultured at room and open field, the other is regenerants cultured in vitro. The results give a new insight for understanding the dynamics of organogenesis in monocot plant.

Keywords
Genetic similarity matrix (GSM)
In vitro organogenesis
Organogenesis-related genes
Plant embryology
Regenerants
Somaclonal variation
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 36
  • No :5
  • Pages :496-507
  • Received Date : 2023-06-07
  • Revised Date : 2023-08-17
  • Accepted Date : 2023-08-31
  • DOI :https://doi.org/10.7732/kjpr.2023.36.5.496
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