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2018 Vol.31, Issue 1 Preview Page

Research Article

Evaluation of Anti-oxidative, Anti-thrombin, Anti-invasive and Pro-apoptotic Activities of Paeonia japonica

백작약(Paeonia japonica)의 항산화, 트롬빈 저해, 암전이 억제 및 암세포사멸 평가

Jun-Ho Kim1
,
Eun-Jung Kim2*
김 준호1
,
김 은정2*
1Department of Fine Chemistry and New Materials, Sangji University
2Department of Biomedical Laboratory Science, Sangji University
1상지대학교 정밀화학신소재학과
2상지대학교 임상병리학과
* Corresponding Author
28 February 2018. pp. 16-23
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Abstract

Paeonia japonica is a perennial flowering plant used in traditional medicine therapy. The purpose of this study was to investigate the effect of water extract and solvent fractions obtained from P. japonica on anti-oxidative, anti-thrombin, anti-invasive and pro-apoptotic activities in YD-10B cells, human oral squamous carcinoma cell line. Water fraction revealed the highest extraction yield at 11.44% (w/w). Anti-oxidative activity was the highest in ethyl acetate fraction (85.13%). In the thrombin inhibitory activity test, ethyl fraction was the highest, with a value of 87.54%. Release and activation of MMP-2/pro-MMP-2 ratio in thrombin-treated YD-10B cells were significantly inhibited in the ethyl acetate fraction. At a concentration of 120 ㎍/㎖, water extract and solvent fractions of P. japonica inhibited cell proliferation in YD-10B cells except water fraction. Pro-apoptotic effect on human oral squamous carcinoma cell using the Bax/Bcl-2 ratio analysis was higher in water extract than other fractions. These findings suggest that the ethyl acetate fraction of P. japonica potentiates a promising antioxidant, anti-thrombin and anti-invasive agents.

Keywords
Anti-oxidative activity
Anti-thrombin activity
Anti-invasive activity
Paeonia japonica
Pro-apoptotic activity
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 31
  • No :1
  • Pages :16-23
  • Received Date : 2017-08-18
  • Revised Date : 2017-10-28
  • Accepted Date : 2017-11-20
  • DOI :https://doi.org/10.7732/kjpr.2018.31.1.016
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