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Research Article

Antioxidants and Acetyl-cholinesterase Inhibitory Activity of Solvent Fractions Extracts from Dendropanax morbiferus

황칠나무의 용매 분획별 추출물의 항산화 활성 및 Acetyl-cholinesterase 저해 활성비교

Ji Min Yu1
,
Hyung In Moon1*
유 지민1
,
문 형인1*
1Department of Medicinal Biotechnology, Dong-A University
1동아대학교 의약생명공학과
* Corresponding Author
28 February 2018. pp. 10-15
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Abstract

The leaves and stems of Dendropanax morbiferus were separated from organic solvents with methanol. The organic solvent fractions were fractionated with dichloromethane, ethyl acetate and butanol according to the systematic fractionation method. Oxidation in the body induces aging, and antioxidant activity has attracted the attention of many people as a preventive component to suppress negative reactions in the body. To investigate the antioxidant activity of Dendropanax morbiferus were subjected to DPPH free radical assay. In addition, acetyl cholinesterase inhibitions were performed for Alzheimer’s disease as an aging neurological disease. As a result, it was confirmed that the antioxidant effect of DPPH was generally good in the antioxidant test. The ethyl acetate fractions of Dendropanax morbiferus stems and leaves were IC50=30 ㎍/㎖. Acetyl cholinesterase inhibition experiments were carried out at a concentration of 250 ㎍/㎖. Dendropanax morbiferus stems fractions showed dichloromethane fraction of 57.68%, which significantly inhibited the activity of acetyl cholinesterase.

Keywords
Acetylcholinesterase inhibitory activity
Dendropanax morbiferus
Free radical scavenging
References
1
Abbott, N.J., L. Ronnback and E. Hansson. 2006. Astrocyte-endothelial interactions at the blood-brain barrier. J. Nat Rev Neurosci. 7:41.
10.1038/nrn182416371949
2
Albertazzi, P., S.A. Steel, E. Clifford and M. Bottazzi. 2002. Attitudes towards and use of dietary supplementation in a sample of postmenopausal women. J. Climacteric. 5:374-382.
10.1080/cmt.5.4.374.382
3
Branen, A.L. 1975. Toxicology and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene. J. American Oil Chem. Soc. 52:59.
10.1007/BF02901825805808
4
Choe, S.Y and K.H. Yang. 1982. Toxicological studies of antioxidants, butylated hydroxytoluene (BHT) and butylated hydroxyanisole (BHA). Korean J. Food Sci. Technol. 14:283-288.
5
Choi, J.H., K.W. Kim, S.E. Park, H.J. Lee, K.M. Kim, K.J. Kim, M.K. Kim, S.J. Kim and S. Kim. 2015. Anti-thrombotic effect of rutin isolated from Dendropanax morbifera leveille. J. Biosci Bioeng. 120:181-186.
10.1016/j.jbiosc.2014.12.01225777266
6
Ellman, G.L., K.D. Courtney, V. Andres and R.M. Featherstone. 1961. A new and rapid colorimetric determination of acetylcholinesterase activity. J. Biochem. Pharmacol. 7:88-95.
10.1016/0006-2952(61)90145-9
7
Fang, Y.Z., S. Yang and G. Wu. 2002. Free radical antioxidant and nutrition. Nutr. 18:872-879.
10.1016/S0899-9007(02)00916-4
8
Gadow, A., E. Joubert and C.F. Hansmann. 1997. Comparison of the antioxidant activity of aspalathin with that of other plant phenols of rooibos tea (Aspalathus linearis), r-Tocopherol, BHT, and BHA. J. Agric. Food Chem. 45:632-638.
10.1021/jf960281n
9
Geldmacher, D.S. 2007. Treatment guidelines for alzheimer’s disease: redefining perceptions in primery care. primary care companion. J. Clin Psychiatry 9:113.
10.4088/PCC.v09n0205
10
Kim, H.J and S.H. Kang. 2017. Ethnobotany, phytochemistry, pharmacology of the Korean campanulaceae: a comprehensive review. Korean J. Plant Res. 30:240-264.
10.7732/kjpr.2017.30.2.240
11
Kim, J.M., S.K. Park, T.J. Guo, J.Y. Kang, J.S Ha, D.S. Lee, O. Kwon, U. Lee and H.J. Heo. 2016. Neuronal cell protective effect of Dendropanax morbifera extract against high glucose-induced oxidative stress. J. Korean Soc. Food Sci. Nutr. 45:938-947 (in Korean).
10.3746/jkfn.2016.45.7.938
12
Lee, D.J. and J.Y. Lee. 2004. Antioxidant activity by DPPH assay. Korean J. Crop Sci. 49:187-194.
13
Musial, A., M. Bajda and B. Malawska. 2007. Recent developments in cholinesterases inhibitors for alzheimer’s disease treatmenr. Curr. Med. Chem. 14:2654-2679.
10.2174/09298670778202321717979717
14
Mo, J.H. and S.J. Oh. 2013. Tyrosinase inhibitory activity and melanin production inhibitory activity of the methanol extract and fractions from Dendropanax morbifera lev. Asian J. Beauty and Cosmetol. 11:275-280 (in Korean).
15
Nakamura, M., J.H. Ra and J.S Kim. 2016. The comparative analysis of antioxidant and biological activity for the Dendropanax morbifera lev. leaves extracted by different ethanol concentrations. J. Pharmaceutical Soc. Japan. 136:1285-1296.
10.1248/yakushi.16-0001827592831
16
Spencer, B., E. Rockenstein, L. Crews, R. Marr and E. Masliah. 2007. Novel strategies for alzheimer’s disease treatment. Expert opin. Biol. Ther. 7:1853-1867.
10.1517/14712598.7.12.185318034651
17
Seow, D. and S. Gauthier. 2007. Pharmacotherapy of alzheimer disease. Can. J. Psychiatry. 52:620-629.
10.1177/07067437070520100318020110
18
Son, H.J. and G.H. Ryu. 2009. Chemical compositions and antioxidant activity of extract from a extruded white ginseng. J. Korean Soc. Food Sci. Nutr. 38:946-950 (in Korean).
10.3746/jkfn.2009.38.7.946
19
Wagner, H. and S. Bladt. 1996. Plant Drug Analysis. A Thin Layer Chromatography Atlas- Alkaloid Drugs. Science & Business Media, München, Germany. pp. 3-51.
Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 31
  • No :1
  • Pages :10-15
  • Received Date : 2017-09-29
  • Revised Date : 2018-01-22
  • Accepted Date : 2018-01-25
  • DOI :https://doi.org/10.7732/kjpr.2018.31.1.010
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