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2022 Vol.35, Issue 5 Preview Page

Research Article

The Induction of Somatic Embryogenic Callus from Petals-Derived Callus in Rosa hybrida

국내 육성 장미 품종 꽃잎 유래 체세포배 발생 캘러스 유도

Su Young Lee1*
,
Ju Young Shin2
,
Young Ah Lee2
,
Chang Ho Ahn3
,
Yae Jin Kim4
,
Pil Man Park1
,
Hye Ryun An4
,
Ka Youn Lee5
,
Hyun Hwan Jung5
이 수영1*
,
신 주영2
,
이 영아2
,
안 창호3
,
김 예진4
,
박 필만1
,
안 혜련4
,
이 가연5
,
정 현환5
1Senior Researcher, Floriculture Research Division, National Institute of Horticultural & Herbal Science, Wanju 55365, Korea
2Researcher Assistant, Floriculture Research Division, National Institute of Horticultural & Herbal Science, Wanju 55365, Korea
3Researcher, Garden Plant Nursery Team, Korea Institute of Arboretum Management, Sejong 30106, Korea
4Researcher, Floriculture Research Division, National Institute of Horticultural & Herbal Science, Wanju 55365, Korea
5Post-doc, Floriculture Research Division, National Institute of Horticultural & Herbal Science, Wanju 55365, Korea
1국립원예특작과학원 화훼과, 연구관
2국립원예특작과학원 화훼과, 연구원
3국립세종수목원 정원소재연구실, 대리
4국립원예특작과학원 화훼과, 연구사
5국립원예특작과학원 화훼과, 박사후연구원
*Corresponding Author
1 October 2022. pp. 652-658
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Abstract

This study was conducted to induce somatic embryogenic callus (SEC) derived from petals in rose. The petal explants of 3 cultivars (‘Ice Wing’, ‘Orange Eye’ and ‘Pink Beauty’) with different flower colors were placed on three types media (MS, SH and WPM) supplemented with 11 ㎎/L 2,4-D, respectively, and then cultured in the dark for 47 days. Calluses were formed at explants of all three cultivars. Also, ‘Ice Wing’, which were cultured in the SH as the basal medium, showed the highest callus formation rate. However, somatic embryos were generated from only petal-derived callus of ‘Ice Wing’, which were induced on the WPM as the basal medium, transferred it to SH basal medium supplemented with 3 ㎎/L 2,4-D, and 300 ㎎/L L-proline, and cultured for 5 weeks. The SEC has been proliferated every four weeks at the subculture interval. In addition, as a results of making a comparison of expression of RhSERK3 and RhSERK4, which is used as signal for generation of somatic embryo from callus in rose, between the SEC and petal-derived callus from ‘Ice Wing’ by RT-qPCR, the former showed 10 times higher RhSERK3 expression and 700 times higher RhSERK4 expression than the latter.

Keywords
Callus, Petals
Rosa hybrida (rose)
Somatic embryogenic callus (SEC)
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 35
  • No :5
  • Pages :652-658
  • Received Date : 2022-07-15
  • Revised Date : 2022-09-05
  • Accepted Date : 2022-09-06
  • DOI :https://doi.org/10.7732/kjpr.2022.35.5.652
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