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2022 Vol.35, Issue 4 Preview Page
Immunostimulatory and Anti-Obesity Activity of Lonicera insularis Nakai Extracts in Mouse Macrophages RAW264.7 Cells and Mouse Adipocytes 3T3-L1 Cells

섬괴불나무(Lonicera insularis Nakai) 추출물의 면역자극 및 항비만 활성

Yu Ju Hyeong1
,
Joo Ho Yeo1
,
Min Yeong Choi1
,
Jae Won Lee2
,
Na Gyeong Geum1
,
Mi-Yun An3
,
Jin Boo Jeong4*
유 주형1
,
여 주호1
,
최 민영1
,
이 재원2
,
금 나경1
,
안 미연3
,
정 진부4*
1Graduate Student, Department of Medicinal Plant Resources, Andong National University, Andong 36729, Korea
2Post-doc, Department of Medicinal Plant Resources, Andong National University, Andong 36729, Korea
3Researcher, National Forest Seed and Variety Center, Chungju 27495, Korea
4Professor, Department of Medicinal Plant Resources, Andong National University, Andong 36729, Korea
1국립안동대학교 생약자원학과, 대학원생
2국립안동대학교 생약자원학과, 박사후연구원
3국립산림품종관리센터, 연구원
4국립안동대학교 생약자원학과, 교수
*Corresponding Author
1 August 2022. pp. 417-427
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Abstract

In this study, we investigated in vitro immuno-stimulatory and anti-obesity activity of fruit (LIF), leaves (LIL) and stems (LIS) from Lonicera insularis Nakai in mouse macrophages RAW264.7 cells and mouse pre-adipocytes 3T3-L1 cells. LIF, LIL and LIS increased the production of immunostimulatory factors such as nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α) and activated phagocytosis in RAW264.7 cells. Inhibition of toll-like receptor 2/4 (TLR2/4) partly blocked LIF, LIL and LIS mediated production of immunostimulatory factors. In addition, inhibition of mitogen-activated protein kinases (MAPK) signaling attenuated the production of immunostimulatory factors induced by LIF, LIL and LIS. Based on these results of this study, LIF, LIL and LIS is thought to activate macrophages the production of immunostimulatory factors and phagocytosis through toll-like receptor 2/4 (TLR2/4) and MAPKs signaling pathway. In anti-obesity study, LIF reduced the lipid accumulation in 3T3-L1 cells. LIF increased the protein phosphorylation expressions such as AMP-activated protein kinase (AMPK), hormone sensitive lipase (HSL), adipose triglyceride lipase (ATGL) related to the lipolysis of the adipocytes. In addition, LIF increased the expression of proteins involved in energy metabolism and brown adipose tissues differentiation such as peroxisome proliferator-activated receptor gamma coativator 1α (PGC-1α) and PR domain-containing16 (PRDM16). These results suggest that LIF is involved in lipid accumulation inhibition through expressing the proteins such as lipolysis and differentiation of white adipocytes to brown adipocytes.

Keywords
Adipocytes
Anti-obesity activity
Differetitation
Immuno-stimulatory activity
Lipolysis
Lonicera Insularis Nakai
Macrophages
3T3-L1
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 35
  • No :4
  • Pages :417-427
  • Received Date : 2022-04-11
  • Accepted Date : 2022-06-16
  • DOI :https://doi.org/10.7732/kjpr.2022.35.4.417
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