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2025 Vol.38, Issue 6 Preview Page

Research Article

Antimicrobial Activity of Chamaecyparis obtusa (Siebold & Zucc.) Endl. Xylem Extract against Methicillin-Resistant Staphylococcus aureus
Ju Yeong So1
,
Dae-Ki Joung2
,
Dong Won Shin3
1Researcher, Material Lab, Dankook University, Cheonan 31116, Korea
2Lecturer, Department of Bio-Herbal Medicine Resources, Sunchon National University, Suncheon 57922, Korea
3Professor, Department of Bio-Herbal Medicine Resources, Sunchon National University, Suncheon 57922, Korea
*Corresponding Author
1 December 2025. pp. 649-656
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Abstract

In this study, the antibacterial activity of Chamaecyparis obtusa (Siebold & Zucc.) Endl. Xylem (COX) was investigated. The antimicrobial activity of the EEC was evaluated using minimal inhibitory concentration (MIC) tests, colorimetric methods with the MTT assay, checkerboard dilution tests, and by testing against six standard strains of methicillin-resistant S. aureus and one standard methicillin-susceptible S. aureus (MSSA) strain. Chamaecyparis obtusa (Siebold & Zucc.) Endl. is for 7 strains the minimum inhibitory concentrations (MICs) are in the range of 125-250 ㎍/mL and the FICI values of EEC + AM, EEC + OX and EEC + VC are 0.01-0.03 and 0.04-0.09 and 0.07-0.25 showed an increase in synergy with the checkerboard method performed using MRSA and MSSA strains. When combined together, this effect were dramatically increased. The time-kill curve was measured at OD 600 ㎚ and showed that the number of bacteria was significantly reduced by combining AM, OX and EEC, which dropped below the lowest measurable limit after 48 h. These results demonstrate that Chamaecyparis obtusa (Siebold & Zucc.) Endl. can be a natural antibacterial agent in MRSA.

Keywords
Antimicrobial activity
Chamaecyparis obtusa (Siebold & Zucc.) Endl.
Methicillin-resistant staphylococcus aureus (MRSA)
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 38
  • No :6
  • Pages :649-656
  • Received Date : 2025-08-13
  • Revised Date : 2025-10-23
  • Accepted Date : 2025-10-23
  • DOI :https://doi.org/10.7732/kjpr.2025.38.6.649
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